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Scientia Silvae Sinicae ›› 2006, Vol. 42 ›› Issue (12): 33-38.doi: 10.11707/j.1001-7488.20061206

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In Vitro Selection and Identification of Heat-Tolerant Clonal Line Z1-1-1 of Senecio cruentus

Yu Xiaoying1,2,Lu Xiangyang2,Yao Jue1,Qiu Shou1,Li Da1,Chen Yonghua3   

  1. 1.College of Horticulture and Landscape, Hunan Agriculture University Changsha 410128; 2.Laboratory of Biochemistry and Fermentation Engineering, Hunan Agriculture University Changsha 410128; 3. College of Resources and Environment, Central South University of Forestry and Technology Changsha 410004
  • Received:1900-01-01 Revised:1900-01-01 Online:2006-12-25 Published:2006-12-25

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Abstract:

In vitro selection of heat-tolerant clonal lines in Senecio cruentus was studied using tussock plantlets and callus as materials and high temperature and HYP as direct and indirect selection stress respectively. The results showed that : 1) 40 ℃ 16～20 h and 30 mmol·L-1 HYP were proper direct and indirect selection stress pressure respectively. 2) One clonal line Z1-1-1 were obtained through high temperature stress selection, and the regenerated plants of Z1-1-1 were more tolerant to high temperature than their explants source genotype(Z1). 3)After transplanted for 30 d, the regenerated plants of Z1-1-1 were different significantly from their explants source genotype(Z1) in the thickness of leaves, the thickness ratio of palisade tissue to spongy tissue(TPT/TST) and the stomatal index. After artificial pollination,the fruit bearing percentage of adult plant of Z1-1-1 was only 1.0% , and Z1’s was over 85%. 4) After heat stress(40 ℃ 24 h),the heat injury index, content of MDA and electrolyte leakage(IL)of the regenerated plants of Z1-1-1 were lower than those of explants source genotype(Z1), but the POD、SOD activity was higher than that of Z1.

Key words: Senecio cruentus, heat-tolerant clonal line, in vitro selection