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Scientia Silvae Sinicae ›› 2008, Vol. 44 ›› Issue (2): 160-164.doi: 10.11707/j.1001-7488.20080225

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rDNA ITS Analysis of Mycorrhizal Fungi in Cymbidium Plants

Li Lubin1,Hu Tao1,Tang Zheng2,Zhuang Caiyun1,Liu Zhenjing1,Yang Kai2,Peng Zhenhua1   

  1. （1. Key Laboratory of Tree Breeding and Cultivation of SAF Research Institute of Forestry，CAF Beijing 100091；2. Key Laboratory of Agricultural New Technology and Application in Beijing, Beijing Agricultural College Beijing 102206）
  • Received:2007-04-25 Revised:1900-01-01 Online:2008-02-25 Published:2008-02-25

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Abstract: rDNA internal transcribed spacer analysis (rDNA ITS) was used to study diversity of 12 representative mycorrhizal Rhizoctonia strains, which were isolated from Chinese orchids (Cymbidium) distributed in different sites and belonged to different ecologic types. The Blast results indicated that all these strains belonged to Epulorhiza or Tulasnella, which was fully consistent with identification as Epulorhiza with morphological method. Additionally, based on rDNA ITS sequence cluster analysis, dendrogram of Cymbidium mycorrhizal strains showed that the distributed environments and orchids species were two crucial factors that affected the specificityrelation between Chinese orchids (Cymbidium) symbiotic mycorrhizae and hosts.

Key words: Cymbidium, mycorrhizalfungi, rDNA internal transcribed spacer analysis, Epulorhiza, Tulasnella