Abstract:

In this study, we tested the cryopreservation of Bursaphelenchus xylophilus at -80 ℃, and investigated effects of different cryoprotectants on the survival and propagation. Cryopreservation with 15%(V/V) glycerol was found as the best protectant for cryopreservation, followed by 10%(V/V), 20%(V/V) and 25%(V/V)glycerol. The poorest protecting effect was with 1% dimethylsulfoxide. With 15%(V/V) glycerol, the survival rate reached to 36.5% after defrosting, and the nematodes retained normal propagation. There was no significant difference in the survival and propagation after cryopreservation between 3-days and 30-days cryopreservation which indicated that cryopreservation with 15%(V/V) glycerol was suitable for B. xylophilus at -80℃.

Key words: Bursaphelenchus xylophilus, cryopreservation, survival rate, propagation