Scientia Silvae Sinicae ›› 2021, Vol. 57 ›› Issue (7): 92-100.doi: 10.11707/j.1001-7488.20210710
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Yuequ Chen1,2,Qingzhen Liu2,Limei Li2,Yang Zhang2,Jiao Han2,Yong'an Zhang1,*
Received:
2020-12-06
Online:
2021-07-25
Published:
2021-09-02
Contact:
Yong'an Zhang
CLC Number:
Yuequ Chen,Qingzhen Liu,Limei Li,Yang Zhang,Jiao Han,Yong'an Zhang. Screening and Identification of Antagonistic Streptomyces for Biocontrol of Poplar Canker[J]. Scientia Silvae Sinicae, 2021, 57(7): 92-100.
Table 1
Inhibition of strains streptomyces to Botryosphaeria dothidea"
菌株 Strains | 抑菌带宽 Inhibition zone/mm | 菌株Strains | 抑菌带宽 Inhibition zone/mm | 菌株Strains | 抑菌带宽 Inhibition zone/mm | ||
HS1 | 20.66±0.647 15 | HS6 | 17.70±1.453 41 | A-1-14 | 12.08±0.962 70 | ||
HS2 | 19.92±2.305 91 | CX1 | 13.18±0.870 17 | F12 | 14.92±0.472 86 | ||
HS3 | 19.96±1.833 79 | CX2 | 13.94±1.107 61 | F15 | 10.48±0.468 61 | ||
HS4 | 17.30±1.487 14 | CX3 | 14.60±0.603 99 | ||||
HS5 | 15.06±0.554 62 | CX4 | 14.66±1.177 96 |
Table 2
Antimicrobial spectra of antagonistic microorganism and fermented broth about HS1"
病原菌 Pathogens | 抑菌带宽 Inhibition zone/mm | 抑菌直径 Diameter of inhibition zone/mm |
蓝莓枯枝病菌小新壳梭孢Neofusicoccum parvum | 12.28±1.36f | 20.69±1.15f |
杨树烂皮病菌金黄壳囊孢菌Cytospora chrysosperma | 20.30±2.36bc | 40.02±0.59a |
烟草赤星病菌链格孢Alternaria alternata | 17.96±0.78d | 32.80±0.78c |
玉米大斑病菌Exserohilum turcicum | 18.46±0.76cd | 39.77±0.79a |
甜瓜枯萎病菌尖孢镰刀菌Fusarium oxysporium | 15.40±0.93e | 21.28±1.17f |
大豆炭疽病菌Colletotrichum truncatum | 21.20±1.10b | 37.61±1.55b |
蓝莓溃疡病菌葡萄座腔菌Botryosphaeria dothidea | 21.72±1.86b | 39.84±0.67a |
瓜果腐霉病菌Pythium aphanidermatum | 14.94±1.07e | 17.12±0.87g |
水稻恶苗病菌串株镰刀菌Fusarium moniliforme | 14.32±0.38e | 29.15±0.59d |
云杉立枯病菌尖孢镰刀菌Fusarium oxysporium | 20.84±1.87b | 29.90±1.05d |
辣椒炭疽病菌Colletotrichum capsici | 15.64±0.71e | 33.70±0.44c |
茄子褐纹病菌Phomopsis vexans | 24.80±0.89a | 36.28±0.63b |
杨树溃疡病菌葡萄座腔菌Botryosphaeria dothidea | 20.66±0.65b | 40.26±0.51a |
Table 3
Inhibition effect of the strain HS1 against poplar canker"
处理 Treatment | 预防作用Prevention | 治疗作用Curation | |||||
发病率 Rate of disease(%) | 病情指数 Disease indexes | 预防效果 Effect of Prevention (%) | 发病率 Rate of disease(%) | 病情指数 Disease indexes | 预防效果 Effect of Prevention (%) | ||
HS1 | 50.00±8.33bc | 24.99±3.61b | 60.87±5.66a | 41.65±8.35c | 18.05±2.40c | 71.74±3.76a | |
多菌灵Carbendazim | 61.31±9.64b | 31.94±2.40b | 50.00±3.76b | 58.34±8.35bc | 33.33±2.08b | 47.83±3.26b | |
对照组CK | 94.44±9.62a | 63.89±3.19a | — | 94.44±9.62a | 63.89±3.19a | — |
Table 4
Cultural characteristic of the strain HS1"
培养基 Medium | 气生菌丝体 Aerial mycelium | 基质菌丝体 Substrate mycelium | 可溶性色素 Soluble pigment | 丰茂度 Abundance |
高氏一号琼脂 Gause No.1 agar | 蚌肉白 Clam white | 酪黄 Buttery yellow | - | +++ |
葡萄糖天门冬素 Glucose asparagine agar | 荷花白 Lotus white | 蚌肉白 Clam white | - | +++ |
蔗糖察氏 Czaper’s agar | 粉白 Powder white | Ⅰ-3-1′ | - | + |
克氏合成一号琼脂 Carrot No.1 agar | 蚌肉白 Clam white | 豆汁黄 Soy sauce yellow | - | +++ |
马铃薯葡萄糖琼脂 Potato glucose agar | 淡红灰 Light red gray | 风帆黄 Sail yellow | 鲛青 Cyan | +++ |
酵母膏麦芽膏琼脂 Yeast extract malt extract agar | Ⅳ-1-2′ | 豆汁黄 Soy sauce yellow | - | +++ |
淀粉铵琼脂 starch ammonium salt medium | Ⅰ-2-2′ | 莲子白 Lotus seed white | - | +++ |
贝奈特琼脂 Bennett’s agar | 鹿角棕 Antler brown | 鹅掌黄 Liriodendron yellow | - | ++ |
葡萄糖酵母膏琼脂 Glucose yeast extract agar | 荷花白 Lotus white | 软木黄 Cork yellow | - | +++ |
甘油天冬素培养基 Glycerol asparagine agar | 鲛青 Cyan | 珠母灰 Pearlescent gray | Ⅶ-7-1′ | ++ |
甘油精氨酸培养基 Glycerine arginine medium | 荷花白 Lotus white | 粉白 Powder white | - | +++ |
黄豆粉蛋白胨培养基 Soybean powder peptone medium | Ⅳ-2-1′ | 粉白 Powder white | - | +++ |
燕麦粉琼脂 Oatemeal agar | Ⅲ-3-3′ | 香水玫瑰黄 Perfume rose yellow | - | +++ |
淀粉琼脂 Soluble starch agar | 乳白 Milky | 淡蜜黄 Light honey yellow | 鹅掌黄 Liriodendron yellow | ++ |
黄豆粉琼脂培养基 Bean agar medium | Ⅳ-3-2′ | 蚌肉白 Clam white | - | +++ |
Table 6
Utilization of carbon sources of the strain HS1"
碳源利用 Utilization of carbon sources | 丰茂度 Abundance | 碳源利用 Utilization of carbon sources | 丰茂度 Abundance | |
D-木糖D-xylose | +++ | 麦芽糖Amylomaltose | ++ | |
D-果糖D-Fructose | +++ | 棉子糖Raffinose | - | |
甘露醇D-mannitol | - | 鸟嘌呤Guanine | + | |
L-鼠李糖L-Rhamnose | - | 甘氨酸Glycine | + | |
α-乳糖α-lactose | + | L-酪氨酸L-tyrosine | ++ | |
葡萄糖Glucose | +++ | L-阿拉伯糖L-Arabinose | + |
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