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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (2): 42-49.

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Factors Affecting Rooting of Picea abies Shoot Cuttings and Individual Selection with High Rooting Ability

Hu Menghong1,2, Ouyang Fangqun1, Jia Zirui1, Wang Junhui1, Zhang Songzhi2, Ma Jianwei2   

  1. 1. Key Laboratory of Tree Breeding and Cultivation of State Forestry Administration Research Institute of Forestry, CAF Beijing 100091;
    2. Xiaolong Mountain Research Institute of Forestry, Gansu Province Tianshui 741022
  • Received:2013-01-17 Revised:2013-03-04 Online:2014-02-25 Published:2014-03-11
  • Contact: 王军辉

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Abstract:

The rooting ability of Picea abies cuttings, exposed to full-light and auto-interval misting, and the affecting factors were studied with the orthogonal experimental design (L8 (14×22)) in Xiaolong Mountain of Gansu Province. The factors included three hormones (ABT1, IBA and NAA) with four levels (0, 50, 100, 200 mg ·L-1), two types of cutting wood (twig, and twig with 1.5-year-old branch), and two levels of treatment time (1 h and 2 h). The experiment arrangement was blocked with 2 replications in random. With these treatments and conditions, the rooting rate of 823 individuals was monitored. The results showed that the reasonable concentration of ABT1 and IBA had dominant influence on rooting of P. abies shoot cutting, the types of shoot cutting and treatment time had different effects on rooting with different types of hormones. The rooting rate of shoot cutting was significantly different with different levels of ABT1. The treatment of 50 mg ·L-1 ABT1 + twig with branch + 2 h dipping had the maximal rooting rate with 89.0%, and the treatment of 200 mg ·L-1 ABT1 + twig with branch + 2 h dipping had maximal rooting number. IBA concentration itself had no significant influence on rooting ability of the shoot cutting, but the treatment of 50 mg ·L-1 IBA + twig with branch + 2 h dipping, and 100 mg ·L-1 IBA + twig with branch + 1 h dipping had the maximal rooting rate and maximal rooting number, respectively. The high concentration NAA had a negative effect on rooting of the calli, therefore, it is not recommended for application in practice, but low concentration NAA with short time soaking could improve rooting from the bark. The variation range of P. abies rooting ability was 0–100.00%, and the potential of individual selection was great. The rooting properties of P. abies was not correlated to growth properties, thus attention must also be paid to the choice of rooting properties when growth properties of P. abies was selected for clonal selection and cutting orchard construction. There were significantly positive correlations among rooting rate, rooting number and average length of roots. Thus, once rooting rate, an easy measured property, was selected, other properties of rooting would be improved genetically.

Key words: Picea abies, shoot cuttings, rooting ability, affecting factors, genetic ability

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