Abstract:

The larvae of scarce chocolate tip, Clostera anachoreta, were fed with leaves of Bt Nanlin 895 poplar to study effects of Bt protein on the metabolic enzymes of the pest. The results showed that the activities of esterase and carboxylesterase were inhibited by Bt protein. The esterase activity of the larvae reduced after being fed with the transgenic poplar leaves in 4-48 h and the activity of carboxylesterase rose during the initial 4 h but declined after then. The activity of multi-function oxidase in the larvae slightly declined after being fed with the transgenic leaves in 24 h, but the difference was not significant from CK. The activity of glutathione-S-transferase did not significantly change during the initial 36 h, but was inhibited by the Bt protein in 48 h. The activity of acetylcholinesterase was not affected by the feeding. The activity of superoxide dismutase rose in 12 and then declined during 12-48 h. The activity of peroxidase rose during 4-48 h while the activity of catalase declined during 4-12 h and rose from 24-48 h. Therefore, the transgenic poplar inhibited the activities of esterase and carboxylesterase, disturbed the protect enzyme system and had an insecticidal effect of the larvae.

Key words: Bt Nanlin 895 poplar, detoxification enzyme, protect enzyme, acetylcholinesterase