Abstract:

This test was carried out with Eucalyptus camaldulensis in vitro to study the high frequency and synchronous production of shoot tips. As 6-Benzylam inopurine (BA) (0.5-1.0mg/L), naphthalene acetic acid (NAA) (0.25-0.5 mg/L) in the medium increasing, more shorter shoots with smaller leaves and more shoot tips suitable for being encapsulated were produced. 40mg/L adenine (ADE) with 1.0mg/L BA and 0.5mg/L NAA in the medium has some role in controleing the shoots and leaves growth, and is fitable for the shoot tip production. The synchronous production of shoot tips was realized through the culture on 16 days light then 4 days dark. In three successive subcultures, mean 25 shoot tips per the material area (cm²) per subculture (20 days) were achieved in the semisolid medium. The problem of brown exudate in shoot subculture was also researched. The measure to overcome was released.

Key words: Eucalyptus camaldulensis, Shoot tips, Synchronous and High frequency