脱落酸诱导黑荆树悬浮细胞抗冻性

Abstract

Abstract: Role of abscisic acid(ABA) in the induction of freezing tolerance in Acacia mearnsii suspension-cultured cells was studied, which mainly concerned the concentration of exogenous ABA added into medium, the time course for the induction of freezing tolerance,induction of increase in soluble proteins and inhibitory effects of cycloheximide on development of freezing tolerance.The results indicated that Acacia mearnsii suspension-cultured cells could be hardened for 8 days at 25℃ by adding ABA into MS medium. Cells treated with ABA (10mg/L) attained a survival of 83.6% at-6℃, while the survival of the nonhardened(control) was 0.In comparision,the cells treated with ABA increased 5.5 fold more soluble proteins.The biosynthesis of soluble proteins was inhibited by cyclohexomide,and the development of freezing tolerance induced by cold acclimation and ABA was simultaneously prevented.

Key words: Acacia mearnsii, Freezing tolerance, ABA

Wang Jingwen;Jiang Jing. INDUCING FREEZING TOLERANCE OF SUSPENSIONCULTURED CELLS IN ACACIA MEARNSII BY ABSCISIC ACID[J]. , 1994, 30(5): 385-390.

[1]	Chen Tingting, Wang Pengkai, Zhang Jiaji, Shi Jisen, Cheng Tielong, Chen Jinhui. Effects of Combined ABA and ZT Treatment on Somatic Embryogenesis and Development of Liriodendron sino-americanum [J]. Scientia Silvae Sinicae, 2019, 55(3): 64-71.
[2]	Du Guangyue, Zhou Shiyu, Liu Dawei, Zhou Yucheng. Technology to Detect Heat Storage Efficiency of Solid Wood Flooring for Ground with Heating System [J]. Scientia Silvae Sinicae, 2018, 54(11): 7-13.
[3]	Du Guangyue, Zhou Shiyu, Liu Dawei, Liu Xiaoping, Zhou Yucheng. Temperature Control System of Closed Adiabatic Dual Cavity in Floor Thermal Storage Efficiency Detector Based on Limiting Fuzzy PID Algorithm [J]. Scientia Silvae Sinicae, 2018, 54(11): 37-44.
[4]	Zhang Peng, Zhao Tongtong, Li Mingyue, Wu Lingdong, Shen Hailong. Effects of CaCl₂, ABA or Sucrose Pretreatment on Germination of Dormancy Released Seeds of Fraxinus mandshurica Dehydrated at Low Temperature [J]. Scientia Silvae Sinicae, 2017, 53(7): 54-61.
[5]	Li Changlin, Jin Li, Yao Yanxing, Su Fuyuan, Pei Qin, Yan Shoukun. An Elite Variety of Malus micromalus ‘Fly fire’ [J]. Scientia Silvae Sinicae, 2016, 52(9): 158-158.
[6]	Wu Fengzhang, Wang Hexin, Xu Guohui, Zhang Zichuan. Research Progress on the Physiological and Molecular Mechanisms of Woody Plants Under Low Temperature Stress [J]. Scientia Silvae Sinicae, 2015, 51(7): 116-128.
[7]	Zhu Zhenjia, Jiang Chengying, Shi Yanhu, Chen Weiqing, Chen Nianlai, Zhao Mengjiong, Wu Wenjun. Variations of Endogenous Hormones in Lateral Buds of Olive Trees(Olea europaea) during Floral Induction and Flower-Bud Differentiation [J]. Scientia Silvae Sinicae, 2015, 51(11): 32-39.
[8]	Liu Changying, Li Jun, Zhao Aichun, Wang Xiling, Lü Ruihua, Wang Xiaohong, Lu Cheng, Yu Maode. Changes of Anthocyanin and Chlorophyll Content, and Expression Levels of Related Genes during Development Process of Mulberry Fruit [J]. Scientia Silvae Sinicae, 2014, 50(9): 59-66.
[9]	Tian Ji, Zhang Jie, Song Tingting, Lu Yanfen, Yao Yuncong. An Elite Variety Malus Crabapple ‘Hongyadang’ [J]. Scientia Silvae Sinicae, 2014, 50(9): 190-190.
[10]	Zhang Jie, Tian Ji, Song Tingting, Lu Yanfen, Yao Yuncong. An Elite Vairety Malus Crabapple‘Shengyinv’ [J]. Scientia Silvae Sinicae, 2014, 50(8): 180-180.
[11]	Song Tingting, Tian Ji, Zhang Jie, Lu Yanfen, Yao Yuncong. An Elite Variety of Malus Crabapple ‘Fenhongzhu’ [J]. Scientia Silvae Sinicae, 2014, 50(6): 188-188.
[12]	Song Tingting, Zhang Jie, Tian Ji, Lu Yanfen, Yao Yuncong. An Elite Variety of Malus Crabapple‘Zixiazhu’ [J]. Scientia Silvae Sinicae, 2014, 50(5): 160-160.
[13]	Chu Guangming, Wang Mei, Zhang Shuoxin. Spatial Point Patters of Anabasis aphylla Populations in the Proluvial Fan of South Junggar Basin [J]. Scientia Silvae Sinicae, 2014, 50(4): 8-14.
[14]	Guo Wei, Lu Mengzhu. Comparison of NAC Transcription Factor Involved in Secondary Vascular Development between Populus tomentosa and Tobacco [J]. Scientia Silvae Sinicae, 2014, 50(3): 22-30.
[15]	Tian Ji, Zhang Jie, Song Tingting, Lu Yanfen, Yao Yuncong. An Elite Malus Crabapple ‘Suyadang’ [J]. Scientia Silvae Sinicae, 2014, 50(10): 190-190.

INDUCING FREEZING TOLERANCE OF SUSPENSIONCULTURED CELLS IN ACACIA MEARNSII BY ABSCISIC ACID

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments