Abstract: This study deals with the circadian rhythms of sex pheromone production and release in the jujube leafroller moth Ancylis sativa Liu. The methods of extraction of sex pheromone glands (SPG) , vial washing collection and electroantennogram assay were used. The results showed that the sex pheromone production of the female moths were not significantly different in the first six days after adult emergence, and the peak of the content of sex pheromone in the glands was seen around 04:00 hours and the peak time of sex pheromone release was around 07:00 hours. The observation of male moth attracted by virgin female in the field indicated a good coincidence with the sex pheromone production and release in the laboratory.

Key words: Ancylis sativa, Circadian rhythm, Sex pheromone gland, Sex pheromone, Extraction of sex pheromone glands, Vial washing collection, Electroantennogram (EAG)