Abstract: Axillary shoots were induced with modified DKW medium + 6-BA 1.0mg/L from the dormant axillary buds collected from young trees,mature trees and stump sprouts of walnut. The formation of adventitious multiple shoots was observed in the subculture with modified DKW medium + 6-BA 1.0mg/L+IBA 0.01mg/L. The efficiency of shoots multiplication was near 600% in one month. After being treated in the medium with IBA 5.0 mg/L for 7 days then cultured the medium contained activated carbon, but without growth regulators for 20 days, 54% shoots rooted. Somatic embryos were induced in darkness from immature embryos (from the middle of May to the early of June) cultured in the modified DKW medium+6-BA 1.0mg/L for 30 days. The somatic embryos germinated in the germinating medium without growth regulators within 7 days in darkness. Suspension culture was established from the embryogenetic callus of walnut.

Key words: Juglans regia, Bud culture, Immature embryo culture, Suspension culture, Organogenesis, Somatic embryogenesis