白蜡窄吉丁成虫对短时高温的生理响应

doi:10.11707/j.1001-7488.LYKX20210691

Abstract

Abstract:

Objective: This study aims to explore the cause of heat tolerance formation in Agrilus planipennis (emerald ash borer, EAB) adults from the physiological level, so as to provide guidance for revealing the formation mechanism of insect resistance to environmental stress. Method: EAB male and female adults were incubated in different high temperature (30, 33, 37 and 41 ℃) and 25 ℃ (served as the control) conditions for 4, 8 and 12 h, respectively, and the insects were collected to measure the water content, fat content, and glycogen, glucose, trehalose, and protein content. Result: With the increase of temperature and the extension of the duration of high temperature, the contents of water and fat in both male and female adults of EAB showed a downward trend. With temperature increasing, the glycogen content was increased, while the contents of glucose and trehalose increased first and then decreased, reaching the highest level at 33?37 ℃. The contents of glycogen, glucose and trehalose in the male and female adults all generally showed an upward trend with increasing time under high temperature. The protein content in the male and female adults increased with fluctuation with the increase of temperature, but gradually decreased with the extension of high temperature duration. The contents of water, fat and glycogen in EAB male adults were significantly higher than those in the female adults, while the contents of trehalose and protein were relatively lower than those in female adults. Conclusion: Under short-time high-temperature conditions, the fat content, and various sugars and protein contents of EAB adults have changed obviously to some extent. The water and fat contents are less affected by temperature and the treatment duration, while the sugar and protein contents are significantly influenced by temperature and the treatment duration. Many stress-resistant substances, such as sugar and protein, play an important role in regulating the resistance of EAB adults against high temperature conditions.

Key words: emerald ash borer, heat tolerance, high-temperature short-time stress, physiological and biochemical indices

CLC Number:

S718.7

Yingqiao Dang,Xiaoyi Wang,Yanlong Zhang,Ke Wei,Liangming Cao. Physiological Responses of Agrilus planipennis Adults to Short-Time High-Temperature Conditions[J]. Scientia Silvae Sinicae, 2023, 59(2): 112-120.

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固定值 Constant value	比较值 Relative value	雄 Male			雌 Female
固定值 Constant value	比较值 Relative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 297	2.707	0.031	4, 275	8.157	<0.001
8 h	25~41 ℃	4, 297	5.114	0.001	4, 275	1.204	0.309
12 h	25~41 ℃	4, 297	4.446	0.002	4, 275	1.314	0.265
25 ℃	4~12 h	2, 297	0.246	0.782	2, 275	1.068	0.345
30 ℃	4~12 h	2, 297	3.937	0.021	2, 275	1.572	0.210
33 ℃	4~12 h	2, 297	0.378	0.685	2, 275	0.739	0.478
37 ℃	4~12 h	2, 297	9.274	<0.001	2, 275	0.403	0.668
41 ℃	4~12 h	2, 297	0.979	0.377	2, 275	5.590	0.004

固定值 Constant value	比较值 Relative value	雄 Male			雌 Female
固定值 Constant value	比较值 Relative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 200	1.364	0.248	4, 234	16.283	<0.001
8 h	25~41 ℃	4, 200	4.472	0.002	4, 234	3.707	0.006
12 h	25~41 ℃	4, 200	2.129	0.079	4, 234	2.952	0.021
25 ℃	4~12 h	2, 200	1.028	0.360	2, 234	2.815	0.062
30 ℃	4~12 h	2, 200	3.263	0.040	2, 234	1.528	0.219
33 ℃	4~12 h	2, 200	1.451	0.237	2, 234	4.712	0.010
37 ℃	4~12 h	2, 200	2.678	0.071	2, 234	0.260	0.771
41 ℃	4~12 h	2, 200	0.820	0.442	2, 234	1.205	0.302

固定值 Constant value	比较值 Relative value	雄 Male			雌 Female
固定值 Constant value	比较值 Relative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 234	0.984	0.417	4, 252	6.831	<0.001
8 h	25~41 ℃	4, 234	12.481	<0.001	4, 252	0.540	0.706
12 h	25~41 ℃	4, 234	0.869	0.483	4, 252	8.903	<0.001
25 ℃	4~12 h	2, 234	2.515	0.083	2, 252	2.549	0.080
30 ℃	4~12 h	2, 234	11.460	<0.001	2, 252	1.711	0.183
33 ℃	4~12 h	2, 234	5.283	0.006	2, 252	2.723	0.068
37 ℃	4~12 h	2, 234	1.792	0.169	2, 252	1.252	0.288
41 ℃	4~12 h	2, 234	4.552	0.011	2, 252	2.872	0.058

固定值 Constant value	比较值 Comparative value	雄 Male			雌 Female
固定值 Constant value	比较值 Comparative value	df	F	P	df	F	P
4 h	25~41 ℃	4, 246	4.690	0.001	4, 251	2.341	0.056
8 h	25~41 ℃	4, 246	1.028	0.394	4, 251	0.351	0.843
12 h	25~41 ℃	4, 246	0.173	0.952	4, 251	0.109	0.979
25 ℃	4~12 h	2, 246	0.006	0.994	2, 251	2.222	0.111
30 ℃	4~12 h	2, 246	0.028	0.973	2, 251	3.051	0.049
33 ℃	4~12 h	2, 246	1.650	0.194	2, 251	3.361	0.036
37 ℃	4~12 h	2, 246	0.294	0.746	2, 251	0.031	0.970
41 ℃	4~12 h	2, 246	9.541	<0.001	2, 251	2.565	0.079

Physiological Responses of Agrilus planipennis Adults to Short-Time High-Temperature Conditions

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