不同倍性广东桑的花粉形态

doi:10.11707/j.1001-7488.20150409

Abstract

Abstract:

【Objective】 This study was aimed to ploidy and cultivar identification by analysis of morphological characteristics of pollen of Morus atropurpurea in order to provide a scientific basis for mulberry polyploidy breeding. 【Method】 The ultrastructure characters of pollen from 10 diploid and tetraploid M. atropurpurea cultivars were examined by scanning electron microscope(SEM), and the characteristics of pollen morphology among the cultivars were distinguished based on statistics and photographic analyses.【Result】 The pollen grains were small in size and nearly round in shape, its polar axis length was 16.98 μm to 24.46 μm, the equatorial axis length was 15.37μm to 23.86 μm, and P/E value of 1.01 to 1.12. The pollen grains with 1-3 rounded germination aperture, N3P3C4 type according to Erdtman's NPC system. There were statistically significant differences between pollen grains of diploid and tetraploid cultivars in the size of germination aperture, the lengths of equator and polar axials, respectively. The pollen exine was unevenly distributed with composite ornamentation of spinous protuberances-brain stripes, and the ornamentation was different among different cultivars. According to the ornamentation, pollen grains are distinctly divided into four types, i.e. coarse spines granulate-fine cerebroid stripes, coarse spines granulate-coarse cerebroid stripes, smooth spines granulate-fine cerebroid stripes and smooth spines granulate-coarse cerebroid stripes. The density and the size of spinous protuberance showed no significant differences between diploid and tetraploid cultivars. Some infertile pollens appeared as heteromophic and shrivelled were observed in tetraploid mulberry cultivars. 【Conclusion】 Significant differences were found of pollen morphology among the 10 diploid and tetraploid M. atropurpurea cultivars, especially in the size of pollen grains between diploid and tetraploid cultivars, and in the ornamentation among different cultivars. It was suggested that morphological characteristics of pollen grains were very important for ploidy detection and cultivars identification in mulberry.

Key words: Morus atropurpurea, pollen grains, ploidy, ultrastructural morphology

CLC Number:

S718.42

Wang Zhenjiang, Luo Guoqing, Dai Fanwei, Yin Hao, Xiao Gengsheng, Tang Cuiming. Pollen Morphology of Morus atropurpurea with Different Ploidies[J]. Scientia Silvae Sinicae, 2015, 51(4): 71-77.

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Pollen Morphology of Morus atropurpurea with Different Ploidies

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