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Scientia Silvae Sinicae ›› 2015, Vol. 51 ›› Issue (1): 150-156.doi: 10.11707/j.1001-7488.20150118

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Analysis on Dynamic of Volatile Organic Substances Released from Pistacia Chinensis

Wang Rong, Hu Jingjing, Wang Chao, Zhang Zhen, Ge Rui, Guo Xiaojing, Shen Xiang   

  1. State Key Laboratory for Crop Biology College of Horticulture Science and Engineering, Shandong Agricultural University Tai'an 271018
  • Received:2014-04-20 Revised:2014-11-30 Online:2015-01-25 Published:2015-01-23

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Abstract:

[Objective]In order to provide a theory basis for the use of leaf volatiles of Pistacia chinensis, it is necessary to understand its diel fluctuation and seasonal dynamics of volatile organic compounds and analyze its volatile composition and relative content.[Method]Five P. chinensis individuals were randomly selected from an adult P. chinensis plantation in North Campus, Shandong Agricultural University. The healthy compound leaves were collected from the outside canopy receiving uniform sunlight at 9:00, 12:00, 15:00, 18:00 and 21:00 on a sunny day in the spring of 2008 (may), summer (July), the fall (October), respectively. The diel fluctuation and seasonal dynamics of volatile organic compounds from P. chinensis leaves were determined by automatic static headspace and GC-MS (gas chromatography-mass spectrometry) technique. [Result]The released volatile substances mainly contained Monoterpenes of β-cis-ocimene, β-trans-ocimene, D-limonene, 3-carene, α-pinene, and β-myrcene, and a small amount of alcohols, ketones and esters. The terpenes accounted for more than 85% of the total volatiles. During a year, the release of terpene substances had a dynamic trend of summer (93.6%) > fall (89.2%) > spring (85.9%). The species of terpene substances were most in spring (59 kinds), followed by that in summer (52 kinds), and then in autumn (50 kinds). The released quantity of alcohols was most in spring, and followed by that in summer and autumn, and the released amount in summer and autumn was similar; Esters, alkanes and aldehydes were released the least in summer, and the released amounts in spring were more than that in autumn. However, ketone release quantity did not change significantly in different seasons. In terms of several major terpenes, the release quantity of beta-cis-Ocimene in different seasons was as the following: spring> summer> autumn, the released beta-Trans-Ocimene was autumn> spring> summer, the released 3-carene was summer > spring > autumn, while the α-pinene and β-myrcene remained unchanged. During a day, the relative contents of monoterpenes showed a trend of the first increased then decreased, and at 15:00, the released amounts generally reached the highest in the day, with smaller in morning and evening. As for several major terpenes, the release quantity of β-cis-ocimene, β-trans-ocimene and D-limonene reached the most in 15:00, regardless of in spring, summer or autumn; and the α-pinene was released the most at 15:00 in spring and autumn, while it was released the maximum at 12:00 in summer; The release quantity of β-myrcene remained uncharged all the time except for that at 18:00 in spring when it slightly dropped. The release quantity of 3-carene remained unchanged in spring and autumn, while it showed a trend of the first increased then decreased with the peak at 12:00 pm. [Conclusion]Monoterpenes were the main volatile substances released from P. chinensis leaves. The seasonal dynamic trends were summer> autumn> spring, and the diel fluctuation showed a trend of 15:00> 12:00> 18:00> 09:00> 21:00.

Key words: Pistacia chinesis, static-headspace gas chromatography-mass spectrometry, VOCs, releasing dynamics

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