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Scientia Silvae Sinicae ›› 2014, Vol. 50 ›› Issue (11): 82-89.doi: 10.11707/j.1001-7488.20141111

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Control Efficacy of Antifungal Protein AMP to Hybrid Bamboo Blight and It's Effect on the Activities of Defense-Related Enzymes in Hybrid Bamboo

Zhang Lina1, Zhu Tianhui2, Peng Yan2, Zheng Lei2, Zhao Fang3   

  1. 1. College of Food Science, Sichuan Agricultural University Ya'an 625014;
    2. College of Forestry, Sichuan Agricultural University Ya'an 625014;
    3. Provincial Key Laboratory of Forest Protection, Sichuan Agricultural University Ya'an 625014
  • Received:2013-10-21 Revised:2014-07-10 Online:2014-11-25 Published:2014-12-04

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Abstract:

Antifungal protein AMP produced by Streptomyces purpeofuscus YSSPG3 was purified from the fermentation. In order to reveal the anti-disease mechanism of AMP to hybrid bamboo blight, hybrid bamboo seedlings were treated with different concentrations of antifungal protein by spraying foliage or soaking root, and inoculated with or without Arthrinium phaeospermum. The effects of antifungal protein on disease index, and the major defense-related enzymes activities were investigated. The results showed that disease index of the bamboo seedlings treating with 10 times, 20 times, and 50 times dilution of antifungal protein stock solution (360.56 μg·mL-1 antifungal protein in the stock solution) was significantly lower than the no-treatment control. After being induced by the antifungal protein, the defense-related enzymes activities of the seedlings, such as superoxide dismutase (SOD), polyphenol oxidase (PPO), peroxidase (POD), catalase (CAT) and phenylalanine ammonialyase (PAL), had different responses to the blight infection. The enzyme activities in the seedling leaves treated with both inducement by the antifungal protein and challenge inoculation with A. phaeospermum had a greater increase than those only treated with inducement. Regardless of challenge inoculation or not, the activities of SOD, PPO, POD, PPO, CAT and PAL in the leaves induced by the antifungal protein had the positive correlations with the concentration of the induced antifungal protein. Amplitude of sequential variation and increment of POD, PPO, CAT activities induced by soaking root was greater than that induced by spraying leaves; There was no significant difference in SOD and PAL between the two inducement methods. The correlation analysis showed that the PPO, POD and CAT activities induced by spraying leaves with antifungal protein had significant correlation with infection index. The SOD and PAL activities induced by soaking root with antifungal protein had significant correlation with infection index. It is suggested that the defense-related enzymes activities might have internal association with infection index.

Key words: Streptomyces purpeofuscus YSSPG3, hybrid bamboo blight, antifungal protein, defense-related enzymes activities

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