Abstract:

To choose the optimal dose of major drug and explore its antifertility effect, the ingestion coefficient and antifertility action of neem oil granules forrodents' ambisexual sterility were studied. The ingestion coefficient was studied with the concentration of 30%, 40% and 50% of neem oil granules for rodents' ambisexual sterility respectively, and the optimal palatability of pharmaceutical preparation was selected to study its antifertility action on mice. The ingestion coefficients at concentration of 30%, 40% and 50% of neem oil granules for rodents' ambisexual sterility were 0.67,0.54 and 0.25 on male mice, and 0.55, 0.50 and 0.22 on female mice. The antifertility action study showed that antifertility rate at concentration of 30% neem oil granules for rodents' ambisexual sterility was 46.7% and 60% on female and male mice,while it at concentration of 40% neem oil granules for rodents' ambisexual sterility was 66.7% and 80% on female and male mice, respectively. The pharmacodynamics at concentration of 40% of neem oil granules for rodents' ambisexual sterility was showed that the testicle coefficient of treated mice was (1.56±0.54)g/100 g, and was significantly lower than the control group(P<0.05). The treated groups' sperm abnormality rate was 45.22%, which was significantly difference compared with the control group (P<0.01). The pathology study showed that 40% of neem oil granules caused serious damages on testicle, vas deferens, hystera, oviduct and ovary of mice. The 40% principle agent of neem oil granules for rodents' ambisexual sterility had good palatability and significant antifertility effect, thus it could be prospective in rodents' control on the grassland.

Key words: Mus muscula, neem oil granules, ingestion coefficient, antifertility action