Abstract:

The anatomy characteristics of cell wall and lignin distribution in different anatomical regions for 12-month old bamboo species Pseudosasa amabilis was investigated. The lignin distribution was studied by means of optical microscope and confocal laser scanning microscope and the color reaction. In addition, the distribution of lignin in different tissues(fiber, parenchyma and vessel) was investigated by visible-light microspectrophotometry coupled with the Wiesner and Mäule reaction. The cell walls of different tissues were all lignified and the lignin was widely distributed in different tissues, in which there was a difference between tissues and anatomical regions in lignin content. Guaiacyl lignin and syringyl lignin unit could be found in cell wall of fiber, parenchyma and vessel through Wiesner and Mäule reaction and the absorbance peak of spectrum. There was no regular variation in lignin content with different radial location of culms and different location in fiber strands. The lamellation of the fiber cell wall was generally had as alternating broad and narrow layers with alternating low and high concentration of lignin.

Key words: Pseudosasa amabilis, characteristics of cell wall, distribution of lignin, visible-light microspectrophotometry