Abstract:

The cytol development of male gametophyte of Bambusa multiplex was studied by dying with a fluorescence dye of Hoechst 33342 and observing with laser scanning confocal microscope (LSCM). The result showed twice mitoses took place and the mature pollens had three cells with one aperture during the development of the male gametophytes, and the male gametophytes abnormally acted at a frequency of 38% in B. multiplex. With the confocal arrangement, serial optical scanning, and advanced three-dimensional reconstruction of the LSCM, direct and visual display of the three-dimensional pollen architecture was available in this study.

Key words: Bambusa multiplex, development of pollen, laser scanning confocal microscope