百合MDHAR基因的克隆与表达分析

doi:10.11707/j.1001-7488.20100929

Scientia Silvae Sinicae ›› 2010, Vol. 46 ›› Issue (9): 178-181.doi: 10.11707/j.1001-7488.20100929

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Cloning and Expression Analysis of MDHAR from Lilium longiflorum

Chen Li;Xin Haibo;Li Xiaoyan;Li Xiaoxin;Yi Mingfang

College of Agriculture and Biotechnology, China Agricultural University Beijing 100193

Received:2009-08-10 Revised:2009-10-13 Online:2010-09-25 Published:2010-09-25

Abstract

Abstract:

A all-length cDNA of LlMDHAR gene was cloned from Lilium longiflorum cv. ‘White Heaven’by RACE. The gene consisted of 1 502 bp encoding a protein of 434 amino acids. The sequencing indicated that the LlMDHAR gene shared highly homology with other plants. RT-PCR analysis showed that expression of LlMDHAR gene could be detected in the root, bulb and leaf, and the relative high expression occurred in the root and leaf. Under H₂O₂ stress, the expression of LlMDHAR gene was increased with the raising of H₂O₂ concentration.

Key words: Lilium longiflorum, monodehydroascorbate reductase gene (MDHAR), cloning, expression　

Chen Li;Xin Haibo;Li Xiaoyan;Li Xiaoxin;Yi Mingfang. Cloning and Expression Analysis of MDHAR from Lilium longiflorum[J]. Scientia Silvae Sinicae, 2010, 46(9): 178-181.

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Cloning and Expression Analysis of MDHAR from Lilium longiflorum

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