Abstract:

Eight fungal strains were isolated from infested parts of diseased seabuckthorn plants，collected at autumn，spring and summer from 2006 to 2007. According to Koch's postulates，the spores of isolates from the infested parts were used as inoculums for inoculating seedlings and 12-year-old seabuckthorn plants. The same disease symptoms were reproduced with isolates. Three strains were recovered after Koch test. Original isolates and recovered strains were identified as Fusarium sporotrichioides based on morphological characteristics and 18S rDNA sequence analysis (100% nucleotide identity). The pathogen was able to grow on or in the leaf，branch，trunk and root of seabuckthorn plants，and there was difference in pathogenicity between the pathogen strains which grow on or in different parts of plant. This is the first report that F. sporotrichioides is the pathogen of seabuckthorn dry shrink in Heilongjiang Province.

Key words: Hippophae rhamnoides, dry shrink, pathogen identification, Fusarium sporotrichioides