Abstract:

A new canker disease occurred on Populus ×euramericana cv‘74/76’ and P.×euramerican ‘zhonglin 46’in Puyang，Henan Province and Heze，Shandong Province in resent years，and caused severe damage to the poplars. Disease incidence increased year after year. Determination of the pathogen of the disease is in an urgent need. Ninetysix samples of the canker were collected from stands of diseased poplars and 10 isolates were obtained，among which most isolates belonged to genus Fusarium. The isolates were inoculated on both hydroponic stem and potted two years old seedlings of Populus ×euramericana cv ‘74/76’ for 4～5 days at 25 ℃. Reisolation from the lesion formed by the inoculation was the same as the inocula，demonstrating that the isolate Mz01 was a pathogenic strain of Fusarium solani based on its morphological. The sequences of the isolates Mz01 were amplyfied by PCR technique with universal primers of fungi，and the sequences were further compared with that in GenBank. The molecular identification confirmed the same results from morphology identification. This is the first report that Fusarium solani is the pathogen of canker disease in Populus ×euramericana in China.

Key words: Populus ×, euramericana canker disease;pathogen;Fusarium solani