Abstract:

The activities of esterase isoenzyme in Tetranychus viennensis Zacher were tested with Polyacrylamide gel electrophoresis after photo and dark reactivation treated by the chloroform extracts, which was obtained by sohxlet method from the root of Tateges erecta. The results showed that both photo and dark treated group with the chloroform extracts could inhibit the activities of esterase isoenzyme. With the increase in the concentration of the extracts, the number of the isoenzyme lanes, the OD value and the peaks area of the gel's scanning curves reduced gradually. The inhibition potential of photo treated group was obviously significant higher than that of the dark treated group. Neither light group nor dark group, there was not any new isoenzyme band coming out after treated by the extracts. Thus, it seems to be that the chloroform extracts of the root of Tateges erecta have some inhibiting activities to the esterase isoenzyme in Tetranychus viennensis Zacher after treated with light.

Key words: Tagetes erecta, Tetranychus viennensis Zacher, Esterase