Abstract:

Based on introduction and selective breeding of Michelia tsoi, genetic variations of 6 M. tsoi types were determined using ISSR marker assays. 12 primers were screened from 30 primers, and total 134 DNA bands were amplified, 67 of which (50%) were polymorphic. The average number of DNA bands amplified by each primer was 11.167. DNA profiles based on ISSR16, ISSR19 and ISSR2 have revealed potential diagnostic fingerprints and specific bands for various types. The ISSR specific bands are useful for the identification of cultivated germplasm, and testing trueness of cultivars. Key steps of ISSR-PCR analysis techniques and application to testing trueness and purity of M. tsoi types were discussed. A dendrogram was constructed by using UPGMA algorithm.

Key words: Michelia tsoi Dandy, Types identification, ISSR-PCR