Abstract:

This paper reports the method of partitioning and purifying plasma membrane from roots of woody plants of Paulownia by improving the method of preparing plasma membrane by aqueous two-phase partitioning in herbaceous plants. The main improvements are adding ascorbic acid and increasing an amount of insoluble pvpp(polyvinylpolypyrrolidone) from 0.05%～0.1% of herbaceous plants to 0.6% in this homogenization medium, and then again collecting the plasma membrane from the lower phase after the first aqueous two-phase partitioning. Through determining the different kinds of marker enzymes of plasma membranes and organelles, that plasma membrane preparations of high yield and purity were easily obtained in aqueous polymer two-phase systems mainly constituted by 6.3% Dextran T-500 and 6.3% polyethylene glycol 4000. This method lays the foundations for the studies on the relation between the principles of roots absorption ions and growth, development of a woody plant at cellular and molecular level.

Key words: Paulownia, Aqueous two-phase partitioning, Plasma membrane, Marker enzyme, Activity of oxidation-reduction