Abstract:

Fifty-five isolates of S.sapinea were obtained on 16 pines and other 2 conifers from 13 Provinces in China. Mycelial parings of the isolates on PDA produced either an incompatible reaction (most of isolates) in which a reaction color line (dark green) between the two isolates developed in interaction zone, or a compatible reaction (few isolates and same isolates) in which no reaction color line developed. Microscopical observation showed that hyphal anastomosis was not found on the former (with reaction line), but found on the latter (no reaction line). So vegetative compatibility of S.sapinea could be measured by observing development of reaction color line between two isolates.\;Among the 55 isolates, 48 vegetative compatibility groups (VCG_S) were recognized, and each of 44 VCG_S included only 1 isolate, 3 VCG_S included 2 isolates, respectively, and 1 VCG included 5 isolates.

Key words: Sphaeropsis sapinea, Vegetative compatibility, Hyphal anastomosis