不同磁场强度对白蚁运动行为和磁感应受体基因表达量的影响

doi:10.11707/j.1001-7488.LYKX20240415

林业科学 ›› 2025, Vol. 61 ›› Issue (9): 146-154.doi: 10.11707/j.1001-7488.LYKX20240415

• 研究论文 • 上一篇

不同磁场强度对白蚁运动行为和磁感应受体基因表达量的影响

高勇勇^1,²,徐焕^1,²,王文靖³,雷忻¹,张延亿^4,⁵,邓刚^4,⁵,黄求应^2,^3,*()

1. 延安大学黄土高原应用生态陕西省高等学校重点实验室　延安716000
2. 华中农业大学水利部白蚁防治重点实验室　武汉 430070
3. 华中农业大学昆虫资源利用与害虫可持续治理湖北省重点实验室　武汉 430070
4. 中国水利水电科学研究院水利部白蚁防治重点实验室　北京100038
5. 中国水利水电科学研究院流域水循环模拟与调控国家重点实验室　北京100038

收稿日期:2024-07-08 出版日期:2025-09-25 发布日期:2025-10-10
通讯作者: 黄求应 E-mail:qyhuang2006@mail.hzau.edu.cn
基金资助:
国家自然科学基金项目（32170500）；陕西省秦创原引用高层次创新创业人才项目（QCYRCXM-2023-087）；延安大学博士启动项目（YAU202213077）

Effects of Magnetic Field Intensity on the Termite Locomotor Behaviour and the Magnetic Receptor Genes Expressions

Yongyong Gao^1,²,Huan Xu^1,²,Wenjing Wang³,Xin Lei¹,Yanyi Zhang^4,⁵,Gang Deng^4,⁵,Qiuying Huang^2,^3,*()

1. Key Laboratory in Shaanxi Province of Applied Ecology for Loess Plateau, Yan’an University　Yan’an 716000
2. Key Laboratory of Termite Control of Ministry of Water Resources, Huazhong Agricultural University　Wuhan 430070
3. Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University　Wuhan 430070
4. Key Laboratory of Termite Control of Ministry of Water Resources, China Institute of Water Resources and Hydropower Research　Beijing 100048
5. State Key Laboratory of Simulation and Regulation of Water Cycle in River Basin, China Institute of Water Resources and Hydropower Research　Beijing 100038

Received:2024-07-08 Online:2025-09-25 Published:2025-10-10
Contact: Qiuying Huang E-mail:qyhuang2006@mail.hzau.edu.cn

摘要/Abstract

摘要：

目的: 研究变化的地磁场对黑翅土白蚁和黑胸散白蚁运动行为以及2种白蚁磁感应受体基因对不同磁场强度的响应，为阐明白蚁磁感应行为的分子机制提供理论依据。方法: 分别在光照和黑暗条件下测试不同磁场强度（强磁场0.1 mT和消除水平分量磁场）对黑翅土白蚁和黑胸散白蚁工蚁运动行为的影响，同时分析2种白蚁的磁感应受体基因对不同磁场强度的响应。结果: 1）在光照条件下，黑翅土白蚁在消除水平分量磁场中的运动转角和角速度均显著增大，但运动速度和距离无显著差异；在强磁场（0.1 mT）中黑翅土白蚁的运动转角和角速度均无显著变化，但运动速度和距离却显著增大。在黑暗条件下，黑翅土白蚁在消除水平分量磁场中的运动转角、角速度、速度和距离均显著增大，但在强磁场中黑翅土白蚁的运动转角、角速度、速度和距离均无显著差异。2）在光照条件下，黑胸散白蚁在消除水平分量磁场中的运动转角、角速度、速度和距离均显著增大，但在强磁场（0.1 mT）中黑胸散白蚁的运动转角、角速度、速度和距离均无显著变化。在黑暗条件下，黑胸散白蚁在消除水平分量磁场中的运动转角和角速度显著增大，但运动距离和速度无显著差异；在强磁场（0.1 mT）中黑胸散白蚁的运动转角、角速度、速度和距离均无显著变化。3）在光照和黑暗条件下，2种白蚁在强磁场（0.1 mT）中处理后，磁感应受体基因（Cryptochromes 2，Cry2）和MagR的相对表达量均显著高于地磁场处理，但在消除水平分量磁场中处理后，Cry2和MagR基因的相对表达量也均显著低于地磁场处理。结论: 无论在光照还是黑暗条件下，消除水平分量磁场均导致2种白蚁的运动转角和角速度显著增大，说明磁场强度变化可以影响白蚁的运动行为。此外，在强磁场（0.1 mT）中处理后，Cry2和MagR基因表达量显著升高，但在消除水平分量磁场中处理后Cry2和MagR基因表达量显著降低，说明白蚁Cry2和MagR基因可以响应磁场强度的变化，同时这也表明Cry2和MagR与白蚁的磁感应行为调控有关。

关键词: 白蚁, 运动行为, 磁感应, Cry2, MagR

Abstract:

Objective: In this study, an investigation was conducted on the effects of changing geomagnetic fields on the locomotor behaviours of Odontotermes formosanus and Reticulitermes chinensis, and the response of magnetic receptor genes to different magnetic field, in order to provide a theoretical basis for further elucidating the molecular mechanisms of the termite magnetic behaviours. Method: In this study, the effects of different magnetic field strengths (the strong magnetic field of 0.1 mT and elimination of the horizontal component of the magnetic field) on the locomotor behaviours of the worker termite R. chinensis and O. formosanus under light and dark conditions were investigated. The expression levels of two magnetic receptor genes response to different magnetic fields in two termite species were analyzed. Result: 1) Under light condition, the walking turn angle and angular velocity of O. formosanus increased significantly, but walking velocity and distance had no significant difference in the elimination of the horizontal component of the magnetic field. In the strong magnetic field (0.1 mT), walking turn angle and angular velocity of O. formosanus had no significant difference under light, but walking velocity and distance of O. formosanus increased significantly. Under darkness, walking turn angle, angular velocity, velocity and distance of O. formosanus increased significantly in the elimination of the horizontal component of the magnetic field. In the strong magnetic field (0.1 mT), walking turn angle, angular velocity, velocity and distance of O. formosanus had no significant difference. 2) The walking turn angle, angular velocity, velocity and distance of R. chinensis increased significantly in the elimination of the horizontal component of the magnetic field under light. In the strong magnetic field, walking turn angle, angular velocity, velocity and distance of R. chinensis had no significant difference under light. Under darkness, walking turn angle and angular velocity of R. chinensis increased significantly, but walking velocity and distance had no significant difference in the elimination of the horizontal component of the magnetic field. In the strong magnetic field, walking turn angle, angular velocity, velocity and distance of R. chinensis had no significant difference under light. 3) The expressions of (Cryptochromes 2, Cry2) and MagR were significantly increased under strong magnetic field (0.1 mT) treatment compared to geomagnetic field, but under elimination of horizontal component of magnetic field treatment, the expressions of Cry2 and MagR were significantly reduced in the two termite species. Conclusion: Regardless of whether it is under light or dark conditions, elimination of the horizontal component of magnetic field significantly increases the walking turn angle and angular velocity of the two termite species, suggesting that the magnetic field intensity affects the locomotor behaviour of termites. In addition, the expressions of Cry2 and MagR genes significantly increase under strong magnetic field (0.1 mT) treatment, while the expressions of the two genes significantly decrease under the elimination of horizontal component of magnetic field, indicating that Cry2 and MagR genes in termites can respond to the changes of magnetic field strength, implying that Cry2 and MagR genes may be involved in the regulation of magnetic sensing behaviours in termites.

Key words: termites, locomotor behaviour, magnetic sensing, Cry2, MagR

中图分类号:

S763.7

高勇勇,徐焕,王文靖,雷忻,张延亿,邓刚,黄求应. 不同磁场强度对白蚁运动行为和磁感应受体基因表达量的影响[J]. 林业科学, 2025, 61(9): 146-154.

Yongyong Gao,Huan Xu,Wenjing Wang,Xin Lei,Yanyi Zhang,Gang Deng,Qiuying Huang. Effects of Magnetic Field Intensity on the Termite Locomotor Behaviour and the Magnetic Receptor Genes Expressions[J]. Scientia Silvae Sinicae, 2025, 61(9): 146-154.

图/表 4

图1

图2

图3

图4

参考文献 0

	丁　芳, 嵇保中, 刘曙雯, 等. 黑翅土白蚁对松木粉及密黏褶菌腐木粉的觅食行为. 林业科学, 2015, 51 (6): 93- 99.
	Ding F, Ji B Z, Liu S G, et al. Foraging behavior of Odontotermes formosanus (Shiraki) (Isoptera: Termitidae) workers to the Pinus massoniana wood powder and the powder infected by brown rot fungus Gloeophyllum trabeum. Scientia Silvae Sinicae, 2015, 51 (6): 93- 99.
	高勇勇. 2021. 黑翅土白蚁和黑胸散白蚁磁定向行为与磁感应机制研究. 武汉: 华中农业大学.
	Gao Y Y. 2021. Magnetic orientation and magnetoreception mechanisms of the termites Odontotermes formosanus and Reticulitermes chinensis. Wuhan: Huazhong Agricultural University. ［in Chinese］
	黄求应. 2006. 黑翅土白蚁觅食行为学基础及诱杀系统的研究. 武汉: 华中农业大学.
	Huang Q Y. 2006. Study on foraging behavior and baiting system for Odontotermes formosanus (Isoptera: Termitidae). Wuhan: Huazhong Agricultural University. ［in Chinese］
	黄求应, 雷朝亮, 薛　东. 黑翅土白蚁的食物选择性研究. 林业科学, 2005, 41 (5): 91- 95. doi: 10.3321/j.issn:1001-7488.2005.05.015
	Huang Q Y, Lei C L, Xue D. Food choice of the underground termite, Odontotermes formosanus. Scientia Silvae Sinicae, 2005, 41 (5): 91- 95. doi: 10.3321/j.issn:1001-7488.2005.05.015
	唐　硕, 叶雨萌, 王雪佳, 等. 2023. 静磁场暴露对大鼠抑郁样行为及海马DR2和DAT表达的影响. 中国体视学与图像分析, 28(3): 294−301.
	Tang S, Ye Y M, Wang X J, et al. 2023. Effects of static magnetic field exposure on the depression-like behaviorand D2 receptor and DAT expression in hippocampus of rats. Chinese Journal of Stereology and Image Analysis. 28(3): 294−301. ［in Chinese］
	王文晶. 2016. 近零磁场对白背飞虱生长发育、飞行能力及体内能源物质的影响研究. 南京: 南京农业大学.
	Wang W J. 2016. Effects of near zero magnetic field on the development, flight behaviour and energy substrates of white-backed planthopper. Nanjin: Nanjing Agricultural University. ［in Chinese］
	王雪峰. 2019. 鸽子隐花色素ClCry的光磁受体性质研究. 长沙: 国防科技大学.
	Wang X F. 2019. Photoreception and magnetoreception of pigeon Cryptochromes (ClCry). Changsha: National University of Defense Technology
	王　怡, 姜宏健, 刘曙雯, 等. 黑胸散白蚁(等翅目: 鼻白蚁科)肛交哺物质的来源. 林业科学, 2020, 56 (11): 116- 123. doi: 10.11707/j.1001-7488.20201112
	Wang Y, Jiang H J, Liu Y W, et al. Sources of the proctodeal trophallaxis substances of Reticulitermes chinensis (Isoptera: Rhinotermitidae). Scientia Silvae Sinicae, 2020, 56 (11): 116- 123. doi: 10.11707/j.1001-7488.20201112
	张　明, 刘瑞莹, 贺静澜, 等. 近零磁场对褐飞虱翅型分化、趋光性及飞行能力的影响. 昆虫学报, 2019, 62 (1): 82- 90.
	Zhang M, Liu R Y, He J L, et al. Wing-form differentiation, phototaxis and flight performance of the brown planthopper, Nilaparvata lugens ( Hemiptera: Delphacidae) under near-zero magnetic fields. Acta Entomologica Sinica, 2019, 62 (1): 82- 90.
	赵方媛. 2020. 磁场对西方蜜蜂生存能力的影响及磁感应相关基因MagR和Cry的表达分析. 南昌: 江西农业大学.
	Zhao F Y. 2020. Effect of magnetic field on survival and expression annalysis of magnetic receptor gene MagR and Cry in Apis wellifera L. Nanchang: Jiangxi Agricultural University. ［in Chinese］
	Bazalova O, Kvicalova M, Valkova T, et al. Cryptochrome 2 mediates directional magnetoreception in cockroaches. Proceedings of the National Academy of Sciences of the United States of America, 2016, 113 (6): 1660- 1665.
	Brembs B, Christiansen F, Pfluger H J, et al. Flight initiation and maintenance deficits in flies with genetically altered biogenic amine levels. Journal of Neuroscience, 2007, 27 (41): 11122- 11131. doi: 10.1523/JNEUROSCI.2704-07.2007
	Cashmore A R, Jarillo J A, Wu Y J, et al. Cryptochromes: blue light receptors for plants and animals. Science, 1999, 284 (5415): 760- 765. doi: 10.1126/science.284.5415.760
	Chang H, Fu X W, Zhao S Y, et al. Molecular characterization, tissue, and developmental expression profiles of MagR and Cryptochrome genes in Agrotisipsilon (Lepidoptera: Noctuidae). Annals of the Entomological Society of America, 2017, 110 (4): 422- 432. doi: 10.1093/aesa/sax043
	Chang H, Guo J L, Fu X W, et al. Molecular characterization and expression profiles of IscA1 gene in a long-distance migrant, Agrotis segetum. Journal of Asia-Pacific Entomology, 2018, 21 (4): 1299- 1306. doi: 10.1016/j.aspen.2018.09.007
	Damulewicz M, Mazzotta G M. One actor, aultiple roles: the performances of cryptochrome in Drosophila. Frontiers in Physiology, 2020, 11, 99. doi: 10.3389/fphys.2020.00099
	Dreyer D, Frost B, Mouritsen H, et al. The earth’s magnetic field and visual landmarks steer migratory flight behavior in the nocturnal Australian bogong moth. Current Biology, 2018, 28 (13): 2160- 2166. doi: 10.1016/j.cub.2018.05.030
	Fleischmann P N, Grob R, Müller V L, et al. The geomagnetic field is a compass cue in Cataglyphis ant navigation. Current Biology, 2018, 28 (9): 1440- 1444. doi: 10.1016/j.cub.2018.03.043
	Gao Y Y, Huang Q Y, Xu H. Silencing Orco impaired the ability to perceive trail pheromones and affected locomotion behavior in two termite species. Journal of Economic Entomology, 2020, 113 (6): 2941- 2949. doi: 10.1093/jee/toaa248
	Gao Y Y, Wen P, Cardé R T, et al. In addition to cryptochrome 2, magnetic particles with olfactory co-receptor are important for magnetic orientation in termites. Communications Biology, 2021, 4 (1): 1121. doi: 10.1038/s42003-021-02661-6
	Hanzlik M, Heunemann C, Holtkamp-Rötzler E, et al. Superparamagnetic magnetite in the upper beak tissue of homing pigeons. Biometals, 2000, 13, 325- 331. doi: 10.1023/A:1009214526685
	Livak K J, Schmittgen T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2^{− ΔΔCT} method. Methods, 2001, 25 (4): 402- 408. doi: 10.1006/meth.2001.1262
	Maeda K, Henbest K B, Cintolesi F, et al. Chemical compass model of avian magnetoreception. Nature, 2008, 453 (7193): 387- 390. doi: 10.1038/nature06834
	Meister M. Physical limits to magnetogenetics. eLife, 2016, 5, e17210. doi: 10.7554/eLife.17210
	Molina-Montenegro M A, Acuña-Rodríguez I S, Ballesteros G I, et al. Electromagnetic fields disrupt the pollination service by honeybees. Science Advances, 2023, 9 (19): 1455. doi: 10.1126/sciadv.adh1455
	Mouritsen H. Long-distance navigation and magnetoreception in migratory animals. Nature, 2018, 558 (7708): 50- 59. doi: 10.1038/s41586-018-0176-1
	Myklatun A, Lauri A, Eder S H, et al. Zebrafish and medaka offer insights into the neurobehavioral correlates of vertebrate magnetoreception. Nature Communications, 2018, 9 (1): 802. doi: 10.1038/s41467-018-03090-6
	Qin S Y, Yin H, Yang C L, et al. A magnetic protein biocompass. Nature Materials, 2016, 15 (2): 217- 226. doi: 10.1038/nmat4484
	Riveros A, Srygley R. 2008. Do leafcutter ants, Atta colombica, orient their path-integrated home vector with a magnetic compass? Animal Behaviour, 75(4): 1273-1281.
	Shaw J, Boyd A, House M, et al. Magnetic particle-mediated magnetoreception. Journal of the Royal Society Interface, 2015, 12 (110): 20150499. doi: 10.1098/rsif.2015.0499
	Stapput K, Thalau P, Wiltschko R, et al. Orientation of birds in total darkness. Current Biology, 2008, 18 (8): 602- 606. doi: 10.1016/j.cub.2008.03.046
	Tong D D, Zhang L, Wu N N, et al. The oriental armyworm genome yields insights into the long-distance migration of noctuid moths. Cell Reports, 2022, 41 (12): 111843. doi: 10.1016/j.celrep.2022.111843
	Wan G J, Wang W J, Xu J J, et al. Cryptochromes and hormone signal transduction under near-zero magnetic fields: new clues to magnetic field effects in a rice planthopper. PLoS ONE, 2015, 10 (7): e0132966. doi: 10.1371/journal.pone.0132966
	Wan G J, Yuan R, Wang W J, et al. Reduced geomagnetic field may affect positive phototaxis and flight capacity of a migratory rice planthopper. Animal Behaviour, 2016, 121, 107- 116. doi: 10.1016/j.anbehav.2016.08.024
	Wang Y Z, Chen J B, Zhu F, et al. Identification of medaka magnetoreceptor and cryptochromes. Science China-Life Sciences, 2017, 60, 271- 278. doi: 10.1007/s11427-016-0266-5
	Wiltschko R, Munro U, Ford H, et al. Light-dependent magnetoreception: orientation behaviour of migratory birds under dim red light. Journal of Experimental Biology, 2008, 211 (20): 3344- 3350. doi: 10.1242/jeb.020313
	Wiltschko R, Stapput K, Thalau P, et al. Directional orientation of birds by the magnetic field under different light conditions. Journal of the Royal Society Interface, 2010, 7 (2): 163- 177.
	Xu H, Huang Q Y, Gao Y Y, et al. IDH knockdown alters foraging behavior in the termite Odontotermes formosanus in different social contexts. Currtnt Zoology, 2021, 67 (6): 609- 620. doi: 10.1093/cz/zoab032
	Xu J J, Pan W, Zhang Y C, et al. Behavioral evidence for a magnetic sense in the oriental armyworm, Mythimna separata. Biology Open, 2017, 6 (3): 340- 347.
	Xu J J, Zhang Y C, Wu J Q, et al. Molecular characterization, spatial-temporal expression and magnetic response patterns of iron-sulfur cluster assembly1 (IscA1) in the rice planthopper, Nilaparvatalugens. Insect Science, 2019, 26 (3): 413- 423. doi: 10.1111/1744-7917.12546
	Zhan S, Merlin C, Boore J L, et al. The monarch butterfly genome yields insights into long-distance migration. Cell, 2011, 147 (5): 1171- 1185. doi: 10.1016/j.cell.2011.09.052
	Zhang B F, Wang L, Zhan A S, et al. Long-term exposure to a hypomagnetic field attenuates adult hippocampal neurogenesis and cognition. Nature Communications, 2021, 12 (1): 1174. doi: 10.1038/s41467-021-21468-x
	Zhu H S, Yuan Q, Briscoe A D, et al. The two CRYs of the butterfly. Current Biology, 2005, 15 (23): 953- 954. doi: 10.1016/j.cub.2005.11.030

[1]	靳正雅,钱沉鱼,杜澄举,马涛,温秀军,王偲. 白蚁、黏土与生态环境相互作用研究进展[J]. 林业科学, 2023, 59(1): 143-150.
[2]	王怡,姜宏健,刘曙雯,嵇保中,熊佳新. 黑胸散白蚁(等翅目:鼻白蚁科)肛交哺物质的来源[J]. 林业科学, 2020, 56(11): 116-123.
[3]	熊佳新,姜宏健,嵇保中,刘曙雯,王怡. 黑胸散白蚁的替代生殖蚁分化[J]. 林业科学, 2019, 55(10): 38-47.
[4]	候亚会, 严善春, 李志强. 对苯二酚对台湾乳白蚁诱食效果及其诱导代谢的响应[J]. 林业科学, 2018, 54(9): 97-103.
[5]	王亚召, 嵇保中, 刘曙雯, 徐立军, 金明霞, 王怡. 黑翅土白蚁菌圃对采食工蚁的诱食活性[J]. 林业科学, 2018, 54(8): 117-123.
[6]	孙骊珠, 罗兰, 袁忠林. 马缨丹提取物对黄胸散白蚁体内酶活性的影响[J]. 林业科学, 2017, 53(5): 107-115.
[7]	谷岱霏, 严善春, 李志强. 氧化苦参碱对台湾乳白蚁纤维素酶活性的抑制作用[J]. 林业科学, 2017, 53(2): 83-88.
[8]	丁芳, 嵇保中, 刘曙雯, 杨锦锦, 张新慰, 王丽平, 赵正萍, 王亚召. 黑翅土白蚁对松木粉及密黏褶菌腐木粉的觅食行为[J]. 林业科学, 2015, 51(6): 93-99.
[9]	马星霞, 蒋明亮, 王洁瑛. 气候变暖对中国木材腐朽及白蚁危害区域边界的影响[J]. 林业科学, 2015, 51(11): 83-90.
[10]	嵇保中, 刘曙雯, 曹丹丹, 冀士琳, 刘佳佳, 王丽平. 白蚁生殖活动中的化学信息交流[J]. 林业科学, 2014, 50(9): 152-160.
[11]	杨秀好;骆有庆;GreggHenderson;毛立新. 基于雷达遥感技术的土栖白蚁探测[J]. 林业科学, 2012, 48(1): 115-120.
[12]	马星霞;王洁瑛;蒋明亮;李小鹰. 中国陆地木材生物危害等级的区域划分[J]. 林业科学, 2011, 47(12): 129-135.
[13]	眭亚萍鲍滨福孙芳利段新芳杨中平. 壳聚糖铜复合物处理后马尾松的防白蚁效果(英文)[J]. 林业科学, 2008, 44(8): 152-154.
[14]	杨天赐莫建初程家安. 白蚁消化纤维素机理研究进展[J]. 林业科学, 2006, 42(1): 110-115.
[15]	黄求应雷朝亮薛东. 黑翅土白蚁的食物选择性研究[J]. 林业科学, 2005, 41(5): 91-95.

不同磁场强度对白蚁运动行为和磁感应受体基因表达量的影响

Effects of Magnetic Field Intensity on the Termite Locomotor Behaviour and the Magnetic Receptor Genes Expressions

RichHTML

PDF

可视化

摘要/Abstract

引用本文

使用本文

图/表 4

参考文献 0

相关文章 15

编辑推荐

Metrics

本文评价

作者中心

期刊获奖

引证指标

联系方式