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林业科学 ›› 2018, Vol. 54 ›› Issue (9): 97-103.doi: 10.11707/j.1001-7488.20180912

• 论文与研究报告 • 上一篇    下一篇

对苯二酚对台湾乳白蚁诱食效果及其诱导代谢的响应

候亚会1, 严善春1, 李志强2   

  1. 1. 东北林业大学林学院 哈尔滨 150040;
    2. 广东省生物资源应用研究所 广东省野生动物保护与利用公共实验室 广东省动物保护与资源利用重点实验室 广州 510260
  • 收稿日期:2017-04-10 修回日期:2018-07-18 出版日期:2018-09-25 发布日期:2018-09-10
  • 基金资助:
    广东省科技计划项目(2016A030303036);广东省科学院科技发展专项(2017GDASCX-0107)。

Synergistic Effect of Antioxidants and Hydroquinoneas on the Attractants and Induced Metabolism of Formosan subterranean termite, Coptotermes formosanus (Isoptera: Rhinotermitidae)

Hou Yahui1, Yan Shanchun1, Li Zhiqiang2   

  1. 1. College of Forestry, Northeast Forestry University Harbin 150040;
    2. Guangdong Key Laboratory of Animal Conservation and Resource Utilization Guangdong Public Laboratory of Wild Animal Conservation and Utilization Guangdong Institute of Applied Biological Resources Guangzhou 510260
  • Received:2017-04-10 Revised:2018-07-18 Online:2018-09-25 Published:2018-09-10

摘要: [目的]研究重要害虫台湾乳白蚁取食选择行为与取食后的代谢产物差异,以探究抗氧化剂对诱食信息素对苯二酚引诱台湾乳白蚁工蚁取食的增效作用及代谢的影响,为台湾乳白蚁诱食剂的开发提供参考。[方法]本文通过2,6-二叔丁基-4-甲基苯酚(BHT)和茶多酚与对苯二酚的混配,比较其对台湾乳白蚁趋性反应的影响及诱食效果,分析抗氧化剂对白蚁诱食信息素对苯二酚的增效作用;通过运用气相色谱飞行时间质谱联用(GC-TOF-MS),分离鉴定诱食处理组与对照组之间有显著差异的代谢产物。[结果]对苯二酚10ng·cm-2剂量条件下,与茶多酚质量比为1:5的混合处理可以显著提高对台湾乳白蚁的诱食效果,而BHT与对苯二酚混配对台湾乳白蚁的诱食无增效作用;与对照组相比,台湾乳白蚁工蚁取食对苯二酚与茶多酚的诱食剂后,其代谢产物显著改变,麦角固醇等5种代谢产物含量明显下降,蜕皮激素等16种代谢产物的含量显著增加。[结论]茶多酚可作为增效剂提高对苯二酚对台湾乳白蚁的诱食作用;对苯二酚与茶多酚诱食剂对台湾乳白蚁的代谢产物具有显著影响。

关键词: 台湾乳白蚁, 对苯二酚, 茶多酚, BHT, 代谢组学

Abstract: [Objective] Formosan subterranean termite, Coptotermes formosanus Shiraki, is an important termite pest in China. Hydroquinone was a valuable phagostimulating pheromone in termites. This study aimed to explore that the synergism of antioxidants combined with hydroquinone on the feeding attraction and the difference in metabolic profiling compared with control for C. formosanus, and provide reference for the development of attractants in C. formosanus.[Method] In this study, butylated hydroxytoluene (BHT) and tea polyphenols as antioxidants were selected, and the orientation and feeding behavior of C. formosanus workers were analyzed in different ratios of antioxidants and hydroquinone using the method of filter paper containing the mixtures. The gas chromatography time-of-flight mass spectrometry (GC-TOF-MS) was used to assay the metabolic substances of the C. formosanus workers fed on the cellulosic feedstock treated by hydroquinone and tea polyphenols.[Result] The results showed there was no significant synergistic effect with different BHT to hydroquinone ratios, while the mixture between 10 ng·cm-2 hydroquinone and tea polyphenol in the mass ratio of 1:5 had significantly higher phagostimulating effect in no-choice and choice experiments. With GC-TOF-MS, untargeted metabolomic analyses revealed that there were significant differences in the metabolic substances compared 10 ng·cm-2 hydroquinone and tea polyphenol in the mass ratio of 1:5 with control group, and a total of 21 different metabolic compounds were identified under the variables with the VIP (variable importance in the projection) values exceeding 1.0, P-value less than 0.01 (Student's t-test), and similarity values more than 300, in which cycloleucine, beta-Glutamic acid, Gluconic lactone, D-erythronolactone and ergosterol were remarkably down-regulated while others were up-regulated.[Conclusion] It was suggested that tea polyphenols had a more obvious synergistic effect than BHT on hydroquinone to C. formosanus workers, and the metabolic profiling of workers fed on cellulose combined with the hydroquinone showed significant difference compared with the control. Tea polyphenols would be a potential synergistic agent used to develop new type of phagostimulating bait against C. formosanus.

Key words: Coptotermes formosanus, hydroquinone, tea polyphenols, butylated hydroxytoluene, metabolomics

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