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›› 1987, Vol. 23 ›› Issue (2): 138-143.

• 论文及研究报告 • 上一篇    下一篇

油茶炭疽病菌毒素对苯丙氨酸解氨酶活性的刺激作用

王敬文   

  1. 中国林业科学研究院亚热带林业研究所
  • 收稿日期:1984-09-03 修回日期:1900-01-01 出版日期:1987-05-25 发布日期:1987-05-25

THE STIMULATION EFFECT OF TOXIN FROM COLLETOTRICHUM CAMELLIAE MASSE ON PHENGLALANINE AMMONIA LYASE (PAL)

Wang Jingwen   

  1. Subtropic Forest Research Institute, Chinese Academy of Forestry
  • Received:1984-09-03 Revised:1900-01-01 Online:1987-05-25 Published:1987-05-25

摘要: 番通油茶炭疽病菌(Colletotrichum camelliae Massee)培养液含有其产生的毒素物质,毒素能使番茄幼苗发生萎蔫和使油茶叶片产生坏死斑,毒素能刺激油茶叶片、愈伤组织和悬浮细脆的苯丙氨酸解氨酶活性增高,所测的抗病型和感病型油茶的酶活性变化时间进程是相同的,但抗病型酶活性增高的幅度为感病型的2.4倍。酶活性增高是由于酶分子的活化和酶蛋白的重新合成引起的。本文讨论了病原菌毒素在抗病性中所起的作用以及利用苯丙氨酸解氨酶活性的增高幅度作为抗病生化指标的可能性。

Abstract: The study of anthorcnose of Camellia oleifera is of economic and scientific importance. The present paper deals with the effects of toxin produced by Colletotrichum camelliae on PAL, which is a determinant enzyme in secondary metabolism and is closely related to disease resistance.Colletoribhum camelliae secretes a toxic substance into culture medium. The toxic substance causes tomato seedling to wilt and induces Lession fleck on leaves of Camellia oleifera. PAL activities from leaves, callus and suspension cells of Camellia oleifera are stimulated increasingly. The time course in increased PAL activities is the same between susceptible and resistant forms, but the increased extent in resistance is equivalent to 2.4—fold in susceptible form during 44 hr. The increase in PAL activity is the result from activation of inactived-enzyme molecular and re-synthesed enzyme protein. The possible roles of toxin produced by C. camelliae in resistance and possibility to take highness in PAL activity which is induced by the toxin for a biochemical indicator of resistance are discussed.