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林业科学 ›› 2004, Vol. 40 ›› Issue (6): 115-120.doi: 10.11707/j.1001-7488.20040620

• 论文及研究报告 • 上一篇    下一篇

2种牛肝菌的生理学特性及抗盐碱性

梁军1 贾秀贞2 张星耀5 王媛4 Hai Nguyen3   

  1. 中国林业科学研究院森林生态环境与保护研究所,北京100091;加拿大艾伯塔大学可更新资源学院埃德蒙顿,加拿大T6G2E1
  • 收稿日期:2004-04-28 修回日期:1900-01-01 出版日期:2004-11-25 发布日期:2004-11-25

The Physiological Characteristics and Salinity and Alkalinity Resistance of Two Ectomycorrhizal Fungi

Liang Jun1,Jia Xiuzhen2,Wang Yuan4,Zhang Xingyao5,Hai Nguyen3   

  1. Research Institute of Forest Ecology, Environment and Protection, CAF Beijing100091 Department of Renewable Resources, University of Alberta Edmonton,Canada T6G2E1
  • Received:2004-04-28 Revised:1900-01-01 Online:2004-11-25 Published:2004-11-25

摘要:

对美味牛肝菌和褐疣柄牛肝菌在不同培养基、不同梯度的培养温度、不同pH值条件、不同碳(C)源和氮(N)源下的生长特性以及抗盐碱性进行了研究。结果表明:美味牛肝菌适合在MMN培养基上生长,褐疣柄牛肝菌适合在PDA、MMN和MRD上生长;2菌种适生pH值范围为4~9,最适pH值为5~6 ,最适生长温度为2 5~30℃;美味牛肝菌利用最好的C源是葡萄糖和淀粉,而褐疣柄牛肝菌利用最好的C源是葡萄糖;2菌种对供试的有机N源均利用较好,尤以牛肉浸膏为最佳;无机N源中,2菌种对(NH4)2SO4 和KNO3利用较好。抗盐碱性试验表明:2菌种有一定的抗盐碱能力,但pH和盐的双胁迫作用显然对菌丝体生长影响显著,菌丝体干质量下降较为明显。

关键词: 美味牛肝菌, 褐疣柄牛肝菌, 生理学特性, 抗盐碱性

Abstract:

To understand cultural and physiological characteristics of ectomycorrhizal fungi was the key to utilize them in large scale production. In this paper the growth characteristics of two ectomycorrhizal fungi, Boletus edulis and Leccinum scabrum were studied on different media, temperature, pH value, different kinds of carbon and nitrogen, and also the resistance to salinity and alkalinity of the two fungi were investigated. The results showed that B. edulis adapted to grow on MMN media and utilized glucose, starch and beef extract best, and L. scabrum was well to grow on media of PDA, MMN and MRD with glucose and beef extract as the sources of carbon and nitrogen; The two fungi could use (NH4)2SO4 and KNO3 very well;and could be able to grow at a pH range 4~9,and grew best at 5~6;the best growth temperature for them was 25~30 ℃. The test also indicated that they had an ability of tolerance to salinity and alkalinity, but the mycelia dry mass dropped down evidently under a double stresses of pH and salt treatment.

Key words: Boletus edulis, Leccinum scabrum, Physiological characteristic, Salinity and alkalinity resistance