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林业科学 ›› 2012, Vol. 48 ›› Issue (5): 78-80.doi: 10.11707/j.1001-7488.20120512

• 论文 • 上一篇    下一篇

松材线虫的低温冷冻保存

吴佳, 王勇军, 张立钦   

  1. 浙江农林大学林业与生物技术学院 临安 311300
  • 收稿日期:2010-09-15 修回日期:2010-12-31 出版日期:2012-05-25 发布日期:2012-05-25
  • 通讯作者: 张立钦

Cryopreservation of Pinewood Nematode (Bursaphelenchus xylophilus)

Wu Jia, Wang Yongjun, Zhang Liqin   

  1. College of Forestry and Biotechnology, Zhejiang A & F University Lin’an 311300
  • Received:2010-09-15 Revised:2010-12-31 Online:2012-05-25 Published:2012-05-25

摘要:

研究-80 ℃下松材线虫的冷冻保存方法,比较不同冷冻保护剂对松材线虫低温保存的保护效果,以及解冻后松材线虫的繁殖情况。结果表明:15%甘油对松材线虫保护效果最好,解冻后存活率达36.5%,其次为10%甘油、20%甘油、25%甘油,保护效果最差的是1%二甲基亚砜(DMSO);解冻后线虫能够正常生长繁殖,冻存3天和冻存30天的线虫存活率和繁殖无显著性差异。

关键词: 松材线虫, 冷冻保存, 存活率, 繁殖

Abstract:

In this study, we tested the cryopreservation of Bursaphelenchus xylophilus at -80 ℃, and investigated effects of different cryoprotectants on the survival and propagation. Cryopreservation with 15%(V/V) glycerol was found as the best protectant for cryopreservation, followed by 10%(V/V), 20%(V/V) and 25%(V/V)glycerol. The poorest protecting effect was with 1% dimethylsulfoxide. With 15%(V/V) glycerol, the survival rate reached to 36.5% after defrosting, and the nematodes retained normal propagation. There was no significant difference in the survival and propagation after cryopreservation between 3-days and 30-days cryopreservation which indicated that cryopreservation with 15%(V/V) glycerol was suitable for B. xylophilus at -80℃.

Key words: Bursaphelenchus xylophilus, cryopreservation, survival rate, propagation

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