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林业科学 ›› 1996, Vol. 32 ›› Issue (6): 569-573.

• 研究简报 • 上一篇    

用PCR扩增16S rDNA检测泡桐组培苗丛枝病类菌原体*

李江山1,金开璇2,汪跃2,熊耀国3,邱并生4   

  1. 1. 中国林业科学研究院林业研究所 北京 100091
    2. 中国林业研究院森林保护研究所 北京 100091
    3. 中国林业科学研究院林业研究所 北京 100091
    4. 中国科学院微生物研究所 北京 100080
  • 收稿日期:1994-12-13 出版日期:1996-11-25 发布日期:1996-11-25

DETECTION OF MYCOPLASMALIKE ORGANISMS(MLO) OF THE PAULOWNIA WITCHES' BROOM TISSUE CULTURE BY A POLYMERASE CHAIN REACTION AMPLIFIING A SEQUENCE OF 16S rDNA

Jiangshan Li1,Kaixuan Jin2,Yue Wang2,Yaoguo Xiong3,Bingsheng Qiu4   

  1. 1. The reserch institute of forestry CAF Beijing 100091
    2. The reserch institute of forest protection CAF Beijing 100091
    3. The reserch institute of forestry CAF Beijing 100091
    4. Institute of Microbilolgy Academia Sinica Beijing 100080
  • Received:1994-12-13 Online:1996-11-25 Published:1996-11-25

关键词: 泡桐丛枝病病原, MLO 16S rDNA, 通用引物, 1.2kb扩增片段, PCR detection

Abstract:

An oligonucleotide primer set (PaF/PaR) was designed for polymerase chain reaction (PCR) amplification of an approximately 1.2kb fragment DNA from Paulownia Witches' broom(PaWB) MLO on the basis of 16S rRNA sequence from O-MLO. Amplification of a 1.2kb DNA fragment from PaWB-MLO-infected plants DNA in total nucleic acid extrats of the host plant Paulownia spp., but no 1.2kb DNA fragment was observed when healthy DNA was used as a template. The study showed that the PCR method was able todetect PaWB-MLO in as little as 9.4pg of total DNA from infected Paulownia tissue culture plantlets.

Key words: Paulownia witches' broom, MLO 16S rDNA, Universal primers, 1.2kb amplification fragment, PCR detection