Abstract: Tissue cultare experiments were carried out with Populus hopeiensos, P. tomentosa and P. alba, all of sect Leuce Duby. Plantlets were induced from isolated branch tissue of trees about 20-year old. Number of adventitious buds would be increased if the tissue was excised on media consisted of 1/2MS macroelements and full MS microelements supplemented with BAP 0.3mg/1+NAA 0.05mg/1+sugar 2.5%or those supplemented with BAP 0.3mg/1+sugar 2%. The best medium tested for rooting is that of 1/2MS macroelements and full MS microelements, supplemented with NAA 0.02mg/1+sugar 1.5%. Plantlets of these three species were transplanted in the nursery, and grew to a height of about 120cm.