白花泡桐CPP转录因子家族的鉴定及其响应多种逆境的表达模式分析

doi:10.11707/j.1001-7488.LYKX20260105

Abstract

Abstract:

Objective: This study systematically analyzed the molecular characteristics of the CPP gene family in P. fortunei and their functions in stress resistance, aiming to provide theoretical basis and genetic resources for the creation of stress-tolerant Paulownia germplasm and its high-value utilization. Method: Based on the whole-genome data of P. fortunei, the bioinformatics, transcriptomics, and molecular biology approaches were comprehensively used to systematically identify members of the PfCPP gene family, and analyze in-depth their physicochemical properties, gene structures, evolutionary relationships, cis-acting elements, and expression patterns in response to biotic (phytoplasma infection) and abiotic (drought, salt) stresses. RT-qPCR was used to verify the expression trends of key genes, and explore their potential functions by combining subcellular localization and protein-protein interaction prediction. Result: A total of 7 PfCPP genes (PfCPP1–PfCPP7) were identified, which were classified into 3 subfamilies and unevenly distributed on 6 chromosomes. Cis-acting element analysis showed that these genes were enriched in elements related to light response, hormone regulation, and stress response. Synteny analysis indicated that segmental duplication was the main driver of the family expansion. Expression profile analysis revealed that PfCPP members exhibited specific expression divergence under different stresses: phytoplasma infection significantly induced the upregulation of PfCPP2/3/4/5/7; under drought stress, PfCPP1–4 were upregulated while PfCPP5–7 were downregulated; only PfCPP7 was significantly induced under salt stress. RT-qPCR results verified the reliability of the transcriptomic data. Subcellular localization showed that PfCPP4 was localized in the nucleus. Protein-protein interaction prediction and molecular docking results suggested that PfCPP4/7 might exert regulatory functions by interacting with MYB-like transcription factors. Conclusion: This study has systematically identified the PfCPP gene family in P. fortunei and revealed their structural characteristics and evolutionary rules. The differential expression patterns of PfCPP genes under various stresses indicate their important regulatory roles in Paulownia stress responses. They may form regulatory networks by interacting with MYB proteins to coordinately regulate Paulownia growth, development, and stress adaptation.

Key words: Paulownia fortunei, CPP gene family, stress, expression analysis, high-value utilization

CLC Number:

S792.25

Mengyao Zhou,Ge Wei,Yuxuan Fan,Enxi Li,Xuefei Tang,Guoqiang Fan. Genome-Wide Identification of the CPP Transcription Factor Family in Paulownia fortunei and Analysis of Their Expression Patterns in Response to Multiple Stresses[J]. Scientia Silvae Sinicae, 2026, 62(9): 13-27.

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拟南芥A. thaliana
AtCPP1	AT2G20110
AtCPP2	AT3G04850
AtCPP3	AT3G16160
AtCPP4	AT3G22760
AtCPP5	AT3G22780
AtCPP6	AT4G14770
AtCPP7	AT4G29000
AtCPP8	AT5G25790
水稻O. sativa
OsCPP1	LOC_Os01g55580
OsCPP2	LOC_Os02g17460
OsCPP3	LOC_Os03g43730
OsCPP4	LOC_Os04g09560
OsCPP5	LOC_Os05g43380
OsCPP6	LOC_Os05g51040
OsCPP7	LOC_Os06g22670
OsCPP8	LOC_Os07g07974
OsCPP9	LOC_Os08g28214
OsCPP10	LOC_Os12g41210
OsCPP11	LOC_Os12g41230
泡桐P. fortunei
PfCPP1	Pfo01g004380.1
PfCPP2	Pfo03g006650.1
PfCPP3	Pfo07g014910.1
PfCPP4	Pfo11g002630.1
PfCPP5	Pfo18g002590.1
PfCPP6	Pfo18g003730.1
PfCPP7	Pfo19g004140.1

引物Primer	序列Sequence （5'?3'）
PfCPP3-F	GTGGGGTTCGTAGACGTTGT
PfCPP3-R	CGGTAGTGATTTTGGCCCCT
PfCPP4-F	AATCTGGAGGGGGTAAGCCA
PfCPP4-R	GCTCCCGCCATCTCAAAAAC
PfCPP5-F	AACTGCCGCGTCGTAAAATG
PfCPP5-R	GGCGTTATTGCAGGCGATTT
PfCPP6-F	CACCTAATCGGCCTGCTCAA
PfCPP6-R	CAGGTGGCTCGATGATGTGT
PfCPP7-F	CTTGCAACACGCAAGCAGAT
PfCPP7-R	GTCTTGCTGGAGTCATCCCC
PfActin-F	AATGGAATCTGCTGGAAT
PfActin-R	ACTGAGGACAATGTTACC

步骤Steps	温度Temperature/℃	时间Time/s
预变性Pre-denaturation	95	30
变性Transsexual	95	5
退火Annealing	58	10
延伸Extension	72	30
溶解曲线Dissolution curve	机器自定义Machine customisation	机器自定义Machine customisation

序号 No.	基因载体 Gene vector	正向引物序列 Forward primer sequence	反向引物序列 Reverse primer sequence
1	pSAK277-PfCPP4	GTGGATCCAAAGAATTCATGGGGGAGGGGGGTGAG	CTCCTTTACCCATGAATTCCTGATTAGGGGTGAGGGAAGG

基因 ID Gene ID	基因名称 Gene name	染色体 Chromosome	氨基酸数目 Number of amino acid	分子量 Molecular weight/kDa	等电点 pI	总平均亲水系数 GRAVY	亚细胞定位 Subcellular location
Pfo01g004380.1	PfCPP1	Chr01	461	49.92	8.38	–0.68	细胞核Nucleus
Pfo03g006650.1	PfCPP2	Chr03	609	65.89	8.53	–0.67	细胞核Nucleus
Pfo07g014910.1	PfCPP3	Chr07	949	102.80	5.97	–0.58	细胞核Nucleus
Pfo11g002630.1	PfCPP4	Chr11	793	86.49	5.58	–0.65	细胞核Nucleus
Pfo18g002590.1	PfCPP5	Chr18	536	58.87	8.60	–0.60	细胞核Nucleus
Pfo18g003730.1	PfCPP6	Chr18	942	102.33	5.65	–0.62	细胞核Nucleus
Pfo19g004140.1	PfCPP7	Chr19	794	86.71	5.37	–0.70	细胞核Nucleus

Genome-Wide Identification of the CPP Transcription Factor Family in Paulownia fortunei and Analysis of Their Expression Patterns in Response to Multiple Stresses

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组分Component	体积Volume/μL
cDNA	1
2× qPCR Master Mix	10
正向引物Forward primer	0.4
反向引物Reverse primer	0.4
ddH₂O	加到20 Add to 20

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登录号Accession number
SRR11787883
SRR11787894
SRR11787905
SRR11787916
SRR11787922-SRR11787928
SRR11787938
SRR11787942-SRR11787944
SRR11787949
SRR11787960
SRR11787971
PRJNA221355
PRJNA289582