北京松山林地nirK型反硝化微生物群落立地及林型效应

doi:10.11707/j.1001-7488.LYKX20220629

Abstract

Abstract:

Objective: This work aims to explore the traits and influence factors of soil nirK-type denitrifying microbial community in different forests in Songshan, Beijing, providing novel insights into the nitrogen cycling process and environmental change of forest soil ecosystem in the warm temperate forest soil ecosystem of North China. Method: Three typical forest types of Pinus tabuliformis, Populus davidiana and Quercus mongolica were selected in the Songshan Nature Reserve of Beijing. Three standard sample plots (20 m×20 m) were set up in each forest type for investigation. The 0?20 cm surface mixed soil samples were collected to analyze soil properties, and extract total soil microbial DNA, PCR was used to amplify nirK gene coding the key enzyme, nitrate reductase, in the denitrification process. The second generation high-throughput sequencing was used to analyze the composition and diversity of soil denitrifying microbial communities in the different forest types, and to explore the effects of soil properties on the characteristics of denitrifying microbial communities. Pearson’s correlation analysis and redundancy analysis (RDA) were used to examine the soil properties factors influencing the trait of denitrifying microbial community. Result: 1) A total of 993 401 effective sequences with 770 328 high-quality sequences of nirK gene were obtained by high-throughput sequencing from 9 soil samples in P. tabuliformis forest, P. davidiana forest and Q. mongolica forest. There were 7 phyla and 78 genera of nirK gene detected in the soil of three different forest types. Among the identified microorganisms, the dominant bacteria of soil nirK denitrification microorganisms in the three forests were Proteobacteria, with the relative abundance of 50% in the three forest types, and the highest of 58.2% in the P. tabuliformis forest. The dominant genera were Bradyrhizobium, Mesorhizobium and Rhodopseudomonas, with a total relative abundance of more than 50%, among which the core genus was Bradyrhizobium. 2) The analysis of alpha diversity of nirK denitrifying microorganisms showed that the Shannon, Simpson and Chao1 diversity index of nirK-type denitrifying microorganisms in P. tabuliformis forest (7.59±0.56, 0.98±0.01, 2 164.24±214.08) were significantly higher than those in P. davidiana forest (5.23±0.26, 0.89±0.02, 1 650.56±136.69) and Q. mongolica forest (5.76±0.38, 0.93±0.02, 1 621.36±156.70) (P<0.05). Based on Bray-Curtis distance algorithm and PCoA analysis, the difference among soil denitrifying microbial communities in different forest types was greater than that within the community. 3) Redundancy analysis showed that soil alkali hydrolyzable nitrogen, nitrate nitrogen and soil organic matter were the key factors that significantly affected the composition of the nirK-type denitrifying microbial community (P<0.05). Conclusion: There are obvious differences in the composition and diversity of soil nirK-type denitrifying microbial communities among P. tabuliformis forest, P. davidiana forest and Q. mongolica forest in the warm temperate zone of North China. Forest types and soil properties such as alkali hydrolyzable nitrogen, nitrate nitrogen and soil organic matter are important factors affecting the traits of soil nirK-type denitrifying microbial communities.

Key words: warm temperate zone of north China, forest type, nirK gene, denitrifying microorganism, microbial community composition, microbial community diversity

CLC Number:

S718.43

Lin Zhu,Lishui Nie,Ce Shi,Mengyao Huang,Xin Niu,Runzhe Zhang,Zhaode Zhang,Yifan Wei,Dengzhi Wang,Hao Yang,Haoliang Nie,Jiang Wang,HuiJuan Bo. Effects of Soil Properties and Stand Factors on nirK-Denitrifying Microbial Community in Songshan, Beijing[J]. Scientia Silvae Sinicae, 2024, 60(5): 139-150.

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林分类型 Forest stands	海拔 Altitude/m	坡度 Slope/(°)	坡向 Aspect	凋落物厚度 Litter depth/cm	林分密度 Stand density/ (plant·hm^?2)	平均树高 Average height/m	平均胸径 Average DBH/cm	郁闭度 Canopy density
油松林Pinus tabuliformis forest	941	18	东East	7.0	894	18	32	0.80
山杨林Populus davidiana forest	869	21	北North	1.0	992	9	14	0.80
蒙古栎林Quercus mongolica forest	856	23	东北Northeast	3.5	985	7	12	0.80

林型 Forest stands	pH	有机质 SOM/(g?kg^?1)	总氮 TN/(g?kg^?1)	碱解氮 AN/(mg?kg^?1)	有效磷 AP/(mg?kg^?1)	速效钾AK/ (mg?kg^?1)	铵态氮NH₄⁺/ (mg?kg^?1)	硝态氮NO₃^?/ (mg?kg^?1)
油松林Pinus tabuliformis forest	6.3 ± 0.3a	70.3 ± 2.8a	1.6 ± 0.1a	247.0 ± 10.1a	2.8 ± 0.1c	378.0 ± 15.4a	24.4 ± 2.9a	28.7 ± 0.2a
山杨林Populus davidiana forest	5.7 ± 0.2a	12.3 ± 0.4c	0.4 ± 0.01c	158.1 ± 4.5b	8.0 ± 0.2a	110.0 ± 3.1c	18.7 ± 0.5b	8.7 ± 0.3b
蒙古栎林Quercus mongolica forest	5.9 ± 0.2a	33.2 ± 1.4b	0.9 ± 0.03b	68.0 ± 2.8c	3.8 ± 0.2b	174.0 ± 7.1b	21.4 ± 1.1b	10.7 ± 2.2b

林型 Forest stands	Shannon指数Shannon index	Simpson指数Simpson index	Chao1指数Chao1 index
油松林 Pinus tabuliformis forest	7.59 ± 0.56a	0.98 ± 0.01a	2164.24 ± 214.08a
山杨林 Populus davidiana forest	5.23 ± 0.26b	0.89 ± 0.02b	1650.56 ± 136.69b
蒙古栎林 Quercus mongolica forest	5.76 ± 0.38b	0.93 ± 0.02b	1621.36 ± 156.70b

土壤性质 Soil properties	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	β多样性 β diversity
pH	0.68*	?0.33	0.47	0.48
有机质 SOM	0.78*	?0.85**	0.70*	0.58
碱解氮 AN	0.40	?0.85**	0.72*	0.46
全氮 TN	0.78*	?0.42	0.70*	0.58
有效磷 AP	?0.85**	0.86**	?0.70*	?0.52
速效钾 AK	0.75*	?0.86**	0.67*	0.52
铵态氮 NH₄⁺-N	0.90**	?0.71*	0.67*	0.30
硝态氮 NO₃^?-N	0.77*	?0.77*	0.70*	0.44

土壤性质 Soil properties	R²	P
pH	0.37	0.25
有机质 SOM	0.46	0.17
全氮 TN	0.44	0.19
碱解氮 AN	0.92	0.001
有效磷 AP	0.13	0.65
速效钾 AK	0.63	0.06
铵态氮 NH₄⁺-N	0.27	0.38
硝态氮 NO₃^?-N	0.71	0.03

Effects of Soil Properties and Stand Factors on nirK-Denitrifying Microbial Community in Songshan, Beijing

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林分因子 Stand factors	Shannon指数 Shannon index	Simpson指数 Simpson index	Chao1指数 Chao1 index	β多样性 β diversity
平均树高 Average height	0.86**	0.70*	0.82**	0.46
平均胸径 Average DBH	0.88**	0.74*	0.82**	0.49
林分密度 Stand density	?0.91**	?0.81**	?0.82**	?0.53
凋落物厚度 Litter depth	0.90**	0.88**	0.73*	0.57
海拔 Altitude	0.87**	0.72*	0.82**	0.47
坡度 Slope	?0.75*	?0.56	0.77*	0.37

林分因子Stand factors	R²	P
平均树高 Average height	0.89	0.001
平均胸径 Average DBH	0.85	0.001
林分密度 Stand density	0.76	0.018
凋落物厚度 Litter depth	0.46	0.181
海拔 Altitude	0.89	0.001
坡度 Slope	0.94	0.001

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