铵硝比对亚热带树种幼苗氮吸收偏好及吸收根属性的影响

doi:10.11707/j.1001-7488.LYKX20220073

Abstract

Abstract:

Objective: This study aims to clarify the effects of different ammonium to nitrate ratios on nitrogen (N) uptake preference and traits of absorptive roots of coniferous and broadleaf seedlings, so as to provide scientific basis for understanding nutrient acquisition and soil N cycle in subtropical plantations. Method: Two-year-old seedlings of common subtropical tree species, including Pinus elliottii, Pinus massoniana, Michelia maudiae, and Liquidambar formosana, were cultured in sands with addition of three different ammonium to nitrate ratios (15∶1, 8∶8, and 1∶15). The inorganic N uptake rates of plants were measured by using the ¹⁵N isotope tracer technique, and the correlations between the inorganic N uptake rates and the morphological and architectural traits of absorptive roots were analyzed. Result: 1) The inorganic N uptake rates of the seedlings of the four species varied under environments with different ammonium to nitrate ratios. The four species preferred to absorb NH₄⁺-N when ammonium was dominant in the sand culture, but preferred to absorb NO₃^?-N when nitrate was dominant. However, species showed different N uptake when the ratio of ammonium to nitrate was 8∶8. The coniferous species absorbed NO₃^?-N at a rate more than twice that of NH₄⁺-N, but there was no significant difference in the uptake of the two forms of nitrogen by broadleaved species. 2) Ammonium-nitrate ratio had a significant impact on root specific length and specific surface area, but had no effect on diameter, tissue density, branching ratio and branching intensity. 3) The inorganic N uptake rate of the four species seedlings was significantly positively correlated with root branches, and significantly negatively correlated with organ biomass. Conclusion: The N uptake rate of seedlings of four tree species depends on substrate N availability and species specificity in response to contrasting edaphic environment. The two coniferous species obtain nutrients through the trade off between physiology (decreased N uptake rate) and morphology (increased specific root length and decreased diameter). However, the two broadleaved species obtain nutrients by simultaneously strengthening the morphological (increased specific root length and surface area), architectural (increased branching ratio), and physiological (increased NH₄⁺-N uptake rate) traits of absorptive roots. These various nutrient acquisition strategies enable tree species to better adapt to changing environments.

Key words: nitrogen uptake rate, absorptive root, morphological traits, ammonium nitrate ratio

CLC Number:

S718.43

Qianyuan Liu,Yifan Chen,Yanmei Chen,Huimin Wang. Effects of Different Ammonium to Nitrate Ratios on Nitrogen Uptake Preference and Traits of Absorptive Roots of Subtropical Trees Seedlings[J]. Scientia Silvae Sinicae, 2023, 59(2): 48-57.

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References 0

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	铵硝比NH₄⁺∶NO₃^?	高度Height /cm	地径Ground diameter /mm
湿地松Pinus elliottii	15∶1	23.06±1.30	4.81±0.18
	8∶8	22.75±1.16	4.51±0.17
	1∶15	21.00±0.53	4.61±0.12
马尾松 Pinus massoniana	15∶1	26.88±1.37	3.45±0.12
	8∶8	26.88±2.02	3.47±0.26
	1∶15	23.00±1.65	3.13±0.14
深山含笑Michelia maudiae	15∶1	72.19±3.48	6.65±0.34
	8∶8	75.31±3.57	7.17±0.13
	1∶15	68.60±3.91	7.28±0.33
枫香Liquidambar formosana	15∶1	75.33±3.22	5.35±0.26
	8∶8	83.08±3.64	5.06±0.16
	1∶15	80.20±1.66	5.79±0.29

因素 Factors	平均值 Mean Square	自由度 Df	F 值 F value	P 值 P value
树种 Tree species	2040.94	3	136.05	< 0.001
铵硝比 NH₄⁺/NO₃^?	169.29	2	11.29	< 0.001
氮形态 N form	250.62	1	16.71	< 0.001
树种×铵硝比 Tree species × NH₄⁺/NO₃^?	96.98	6	6.47	< 0.001
树种×氮形态 Tree species × N form	73.44	3	4.90	0.004
铵硝比×氮形态 NH₄⁺/NO₃^? × N form	2785.03	2	185.65	< 0.001
树种×铵硝比×氮形态 Tree species × NH₄⁺/NO₃^? × N form	432.43	6	28.83	< 0.001
误差 Error	15.00	72

P值 P value因素 Factor	直径Diameter	比根长Specific root length	比表面积Specific surface area	组织密度Root tissue density	分支比Branching ratio	分支强度 Branching intensity
铵硝比 NH₄⁺/NO₃^?	0.68	0.023	0.022	0.463	0.053	0.410
树种 Tree species	< 0.001	< 0.001	< 0.001	0.001	< 0.001	< 0.001
树种×铵硝比 Tree species × NH₄⁺/NO₃^?	0.515	0.030	0.045	0.429	0.905	0.708

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Effects of Different Ammonium to Nitrate Ratios on Nitrogen Uptake Preference and Traits of Absorptive Roots of Subtropical Trees Seedlings

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