麻竹转录因子DlSCL6的基因克隆及在拟南芥中异位表达

doi:10.11707/j.1001-7488.20141107

Abstract

Abstract:

HAM (Hairy Meristem) subfamily in GRAS family is a group of transcription factors that play important roles in plant growth and morphogenesis. By using RT-PCR and RACE technique, a HAM homologous gene, DlSCL 6 was cloned from Dendrocalamus latiflorus. This gene has 2 006 bp in full-length including the 5' untranslated region (UTR) of 129 bp, 3' UTR of 266 bp, and coding region of 1 611 bp. DlSCL6 protein has five conserved domains (LHR Ⅰ, VHIID, LHR Ⅱ, PFYRE, and SAM), and is relatively high consistent to SCL6 proteins in some monocots, and some consistent of 43% to the Arabidopsis thaliana. A recombinant protein with approximately 60 kDa was obtained in the expression of this gene in Escherichia coli. The sense transgenic plants derived from ectopic expression of DlSCL 6 in A. thaliana showed prolonged vegetative growth, delayed flowering, and increased rosette leaf number, while the antisense transgenic plants had early flowering and thin plants and significantly reduced rosette leaves number. This study indicates that DlSCL 6 gene can influence the shoot apical meristem status, and hence result in changes in morphogenesis.

Key words: Dendrocalamus latiflorus, transcription factor, Arabidopsis thaliana, ectopic expression

CLC Number:

S718.46

Yang Li, Chen Dongliang, Peng Zhenhua, Zhao Hansheng, Gao Zhimin. Cloning of Transcription Factor DlSCL6 from Dendrocalamus latiflorus and Its Ectopic Expression in Arabidopsis thaliana[J]. Scientia Silvae Sinicae, 2014, 50(11): 52-57.

References

陈东亮,彭镇华,高志民. 2013. 毛竹PePsbS 1 基因的分子特征及其原核表达. 安徽农业大学学报,40(1): 5-10.
(Chen D L, Peng Z H, Gao Z M. 2013. Molecular characteristics and prokaryotic expression of PePsbS1 gene from Phyllostachys edulis. Journal of Anhui Agricultural University,40(1): 5-10. [in Chinese] )
崔丽莉,杨汉奇,杨宇明. 2010. 版纳龙竹CONSTANS同源基因的克隆与序列分析. 林业科学研究,23(1): 1-5.
(Cui L L, Yang H Q, Yang Y M. 2010. Cloning and sequence analysis of CONSTANS homologous gene from Dendrocalamus xishuangbannaensis. Forest Research,23(1): 1-5. [in Chinese] )
高志民,郑波,彭镇华. 2010. 毛竹MADS-box基因的克隆及功能初步研究. 林业科学,46(10): 37-41.
(Gao Z M, Zheng B, Peng Z H. 2010. Isolation of PeMADS1 gene from Phyllostachys edulis and its transformation in Arabidopsis thaliana. Scientia Silvae Sinicae,46(10): 37-41. [in Chinese] )
邱尔发,郑郁善,洪伟,等. 2002. 开花和未开花山地麻竹叶片性状比较. 福建林学院学报,22(2): 105-108.
(Qiu E F, Zheng Y S, Hong W, et al. 2002. A comparison of leaves character between flowering and no-flowering Dendrocalamus latiflorus planted on mountain. Journal of Fujian College of Forestry,22(2): 105-108. [in Chinese] )
田波,陈永燕,严远鑫,等. 2005. 一个竹类植物MADS盒基因的克隆及其在拟南芥中的表达. 科学通报,50(2): 145-151.
徐芳,熊爱生,彭日荷,等. 2005. 植物遗传转化的新方法: Floral Dip. 中国蔬菜,1(3): 29-31.
(Xu F, Xiong A S, Peng R H, et al. 2005. Floral dip: a new method in genetic transformation of plant. China Vegetables,1(3): 29-31. [in Chinese] )
周本智. 1999. 麻竹出笋和高生长规律的研究. 林业科学研究,12(5): 461-466.
(Zhou B Z. 1999. Rule of shooting and height-growth of Dendrocalamus latiflorus. Forest Research, 12(5): 461-466. [in Chinese] )
Bolle C, Koncz C, Chua N H. 2000. PAT1, a new member of the GRAS family, is involved in phytochrome A signal transduction. Gene Dev, 14(10): 1269-1278.
Bolle C. 2004. The role of GRAS proteins in plant signal transduction and development. Planta, 218(5): 683-692.
Deléage G, Blanchet C, Geourjon C. 1997. Protein structure prediction. Implications for the biologist. Biochimie, 79(11): 681-686.
Engstrom E M, Andersen C M, Gumulak-Smith J, et al. 2011. Arabidopsis homologs of the petunia hairy meristem gene are required for maintenance of shoot and root indeterminacy. Plant Physiol, 155(2): 735-750.
Fode B, Siemsen T, Thurow C, et al. 2008. The Arabidopsis GRAS protein SCL14 interacts with class Ⅱ TGA transcription factors and is essential for the activation of stress-inducible promoters. Plant Cell, 20(11): 3122-3135.
Gao Z M, Li X P, Li L B, et al. 2006. An effective method for total RNA isolation from bamboo. Chinese Forest Sci Tech, 5(3): 52-54.
Gao Z M, WangX C, Peng Z H, et al. 2012. Characterization and primary functional analysis of phenylalanine ammonia-lyase gene from Phyllostachys edulis. Plant Cell Rep, 31(7): 1345-1356.
Greb T, Clarenz O, Schafer E, et al. 2003. Molecular analysis of the LATERAL SUPPRESSOR gene in Arabidopsis reveals a conserved control mechanism for axillary meristem formation. Genes Dev, 17(9): 1175-1187.
He C Y, Cui K, Zhang J G, et al. 2013. Next-generation sequencing-based mRNA and microRNA expression profiling analysis revealed pathways involved in the rapid growth of developing culms in Moso bamboo. BMC Plant Biol, 13:119.
Hisamoto Y, Kashiwagi H, Kobayashi M. 2008. Use of flowering gene FLOWERING LOCUS T (FT) homologs in the phylogenetic analysis of bambusoid and early diverging grasses. J Plant Res, 121(5): 451-61.
Kyte J, Doolittle R F. 1982. A simple method for displaying the hydropathic character of a protein. J Mol Biol, 157(1): 105-132.
Lee M H, Kim B, Song S K, et al. 2008. Large-scale analysis of the GRAS gene family in Arabidopsis thaliana. Plant Mol Biol, 67(6): 659-670.
Li X, Qian Q, Fu Z, et al. 2003. Control of tillering in rice. Nature, 422(6932): 618-621.
Ma H S, Liang D, Shuai P, et al. 2010. The salt- and drought-inducible poplar GRAS protein SCL7 confers salt and drought tolerance in Arabidopsis thaliana. J Exp Bot, 61(14): 4011-4019.
Pysh L D, Wysocka-Diller J W, Camilleri C, et al. 1999. The GRAS gene family in Arabidopsis: sequence characterization and basic expression analysis of the SCARECROW-LIKE genes. Plant J, 18(1): 111-119.
Schulze S, Schäfer B N, Parizotto E A, et al. 2010. LOST MERISTEMS genes regulate cell differentiation of central zone descendants in Arabidopsis shoot meristems. Plant J, 64(4): 668-678.
Stuurman J, Jäggi F, Kuhlemeier C. 2002. Shoot meristem maintenance is controlled by a GRAS-gene mediated signal from differentiating cells. Gene Dev, 16(17): 2213-2218.
Torres-Galea P, Huang L F, Chua N H, et al. 2006. The GRAS protein SCL13 is a positive regulator of phytochrome-dependent red light signaling, but can also modulate phytochrome A responses. Mol Genet Genomics, 276(1): 13-30.
Wang L, Mai Y X, Zhang Y C, et al. 2010. MicroRNA171c-targeted SCL 6 -Ⅱ , SCL 6 - Ⅲ , and SCL 6 - Ⅳ genes regulate shoot branching in Arabidopsis. Mol Plant, 3(5): 794-806.
Zhou M B, Yang P, Gao P J, et al. 2011. Identification of differentially expressed sequence tags in rapidly elongating Phyllostachys pubescens internodes by suppressive subtractive hybridization. Plant Mol Biol Rep, 29(1): 224-231.

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Cloning of Transcription Factor DlSCL6 from Dendrocalamus latiflorus and Its Ectopic Expression in Arabidopsis thaliana

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