云杉体细胞胚萌发前的干化标记

doi:10.11707/j.1001-7488.20140705

Abstract

Abstract:

In order to overcome the low germination rate and plantlet quality of Picea asperata, partial desiccation factors, such as drying approaches, drying time and universality, were investigated with highly synchronized somatic embryos, and a crucial partial desiccation indicator was established based on variation in the embryos morphology and color. The results indicated that the "paper vessel method" was more appropriate for the partial desiccation treatment, and the maximum germination rate was obtained with drying treatment for two weeks. Meanwhile, this study provided a drying treatment program which showed a general applicability for somatic embryos of P. asperata. The marked embryos of green cotyledon, hypocotyle and red radical could universally obtain high germination rate(average 83.63%)and good plantlet quality.

Key words: Picea asperata, somatic embryo, germination, partial desiccation indicator

CLC Number:

S718.46

Zhang Jianwei, Wang Junhui, Zhang Shougong, Li Qingfen, Xia Yan. A Desiccation Indicator before Germination of Picea asperata Somatic Embryos[J]. Scientia Silvae Sinicae, 2014, 50(7): 31-36.

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A Desiccation Indicator before Germination of Picea asperata Somatic Embryos

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