纤维素基质固载酶材料制备及固定化漆酶性能

doi:10.11707/j.1001-7488.20131117

Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (11): 122-128.doi: 10.11707/j.1001-7488.20131117

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Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance

Guo Ming¹, Yan Bingyu¹, Wang Chunpeng², Zhou Jianzhong¹

1. Department of Chemistry, Zhejiang Agriculture & Forestry University Lin'an 311300;
2. Institute of Chemical Industry of Forest Products, CAF Nanjing 210042

Received:2012-12-31 Revised:2013-03-12 Online:2013-11-25 Published:2013-11-26

Abstract

Abstract:

Dialdehyde cellulose(DAC), a carrier material of cellulose-based immobilized laccase using microcrystalline cellulose(MCC)as raw materials was prepared by new hydrothermal oxidation synthesis method. The structure was characterized by IR, CP/MAS¹³C NMR, XRD and SEM. The enzymatic characteristics of cellulose-based immobilized laccase were tested and compared with chitosan-based immobilized laccase and dialdehyde starch-based immobilized laccase. The theoretical equations of enzymatic characteristics were established. The experimental results showed that DAC containing high aldehyde group content was prepared successfully using hydrothermal oxidation synthesis method. The dialdehyde cellulose-based immobilized laccase had better affinity ability, higher reusage and maintained enzyme activity within broader temperature and pH regions compared with chitosan-based immobilized laccase and dialdehyde starch-based immobilized laccase. The theoretical equations were well able to characterize the enzymatic characteristics.

Key words: dialdehyde cellulose (DAC), hydrothermal synthesis, immobilized laccase, structure characterization, enzymatic characteristics

CLC Number:

TQ352.79
Q55

Guo Ming, Yan Bingyu, Wang Chunpeng, Zhou Jianzhong. Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance[J]. Scientia Silvae Sinicae, 2013, 49(11): 122-128.

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Preparation of Cellulose-Based Immobilized Enzyme Matrix and the Exploration of Immobilized Laccase Performance

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