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Scientia Silvae Sinicae ›› 2013, Vol. 49 ›› Issue (9): 23-27.

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Separation and Identification of Triterpenic Acids from Suspended Cultured Cells of Cyclocarya paliurus

Yin Zhongping1,2, Shangguan Xinchen1,2, Chen Jiguang1,2, Wu Shaofu2, Li Dongming2   

  1. 1. Jiangxi Key Laboratory of Natural Products and Functional Food Nanchang 330045;
    2. Food Science and Engineering Department, Jiangxi Agricultural University Nanchang 330045
  • Received:2012-09-12 Revised:2013-01-14 Online:2013-09-25 Published:2013-09-19

Abstract:

In this paper, five triterpenic acids were separated from suspended cultured cells of Cyclocarya paliurus by using macroporous resin adsorption, silica gel column chromatography, Sephadex LH-20 gel filtration chromatography, semipreparative liquid chromatography and recrystallization, and identified as (2α,3β)-2,3-dihydroxy-olean-12-en-28-oic acid, (2α,3β)-2,3-dihydroxy-urs-12-en-28-oic acid, betulinic acid, oleanolic acid and ursolic acid by RRLC-TOF/MS, 1H NMR and 13C NMR. The (2α, 3β)-2, 3-dihydroxy-olean-12-en-28-oic acid and betulinic acid were for the first time separated and identified from C. paliurus.

Key words: plant cell culture, Cyclocarya paliurus, triterpenic acid, separation, structure identification

CLC Number: