Abstract:

Steroid saponins? was extracted from the Polygonatum cirrhifolium root.Two compounds were isolated and purified by the combined use of column chromatography with silical gel,RP-18 and SephadexLH-20 methods.The structure of the compound were elucidated by spectroscopic and the data reported in literature methods.Two compounds were identified as(25R)-spirost-5-ene-3β-ol-3-O-α-L-rham-nopyranosy(1→2)-[α-L-rhamnopyranosyl(1→4)]-β-D-glucopyranoside(Ⅰ)and(25R)-spirost-5-ene-3β-ol-3-O-α-L-rhamnopyranosyl(1→4)-β-D-glucopyranoside(Ⅱ).The aglycones of compound Ⅰ was obtained by the method of two-phase acid hydrolysis and its structure was identified as diosgenin(Ⅲ).The antisepsis activity of R,compound Ⅰ,Ⅱ and Ⅲ were studied,the results were as follows:Steroid saponins R,compound Ⅰ and Ⅱ had strong inhibition against plant pathogenic fungi growth than bacteria's,but compound Ⅲ had strong inhibition against to bacteria growth than plant pathogenic fungi's.

Key words: Polygonatum cirrhifolium, steroid saponins, structure identify, antisepsis activity