Abstract:

The leaf disc of Eucalyptus urophylla was regenerated by use of the quadratic orthogonal rotation design to optimize the differentiation medium for the callus.The leaf discs of the E.urophylla were co-cultivated with Agrobacterium tumefaciens carrying the target gene.20 sprouts which resisted to the Kam were obtained after callus inducing and Kam sereening.8 out of them regenerated from the rooting medium E_3-B supplied with the same concentration of Kam can be considered as transgenic seedlings.The nopaline synthetic enzyme activity of the preliminary transformation was detected by electrophoresis.Using the anti-bacterial peptide gene labelled with γ-³²p-dCTP or DIG as probes,the introduced gene was detected in the preliminary transformations through dot blotting and Southern blotting.Innoculation of the preliminary transgenic seedlings with high virulent Pseudomonas solanacearum (991016) caused a death rate of 56.7%,30% lower than that of the control.It was demonstrated from the above results that the anti-bacterial peptide gene was integrated into the genome of Eucalyptus plant and the transgenic plant with higher resistance were obtained.

Key words: Eucalyptus urophylla, Cecropin D, Bacterial-wilt disease, Antheraea pernyi