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Scientia Silvae Sinicae ›› 2026, Vol. 62 ›› Issue (7): 100-112.doi: 10.11707/j.1001-7488.LYKX20250644

• Research papers • Previous Articles     Next Articles

Screening of Endophytic Phosphate-Solubilizing Bacteria from Vernicia fordii and Their Growth-Promoting Effects

Hao Wang1,Yuxiang Guo1,Chen Chen1,Sifan Liu2,Zan Liu2,Gang He2,Nianyuan Liu3,Yanling Zeng1,*()   

  1. 1. State Key Laboratory of Woody Oil Resources Utilization Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education College of Forestry, Central South University of Forestry and Technology Changsha 410004
    2. Forestry Science Research Institute in Xiangxi Tujiazu & Miaozu Autonomous Prefecture Jishou 416000
    3. Forestry Bureau of Xiangxi Tujiazu & Miaozu Autonomous Prefecture Jishou 416000
  • Received:2025-10-27 Online:2026-07-10 Published:2026-07-14
  • Contact: Yanling Zeng E-mail:zengyanling110@csuft.edu.cn

Abstract:

Objective: This study aims to explore functional endophytic bacteria resources from Vernicia fordii (tung tree), screen efficient phosphate-solubilizing strains, so as to provide a scientific basis for developing growth-promoting bacterial agents for plants in rocky desertification regions. Method: Endophytic bacteria were isolated from roots, stems and leaves of healthy tung trees. Phosphate-solubilizing strains were obtained through primary screening by phosphate-solubilizing halo and rescreening by molybdenum-antimony anti-colorimetric method. Strains were identified by 16S rRNA gene sequencing. Their nitrogen-fixation, IAA production, and antagonism against Fusarium oxysporum were analyzed. Pot experiments were conducted to verify their effects on growth and nutrient accumulation in V. fordii/V. montana seedlings treated with phosphate solubilizing bacteria. Result: A total of 31 species of fungi and bacteria were isolated. Burkholderia cepacia (RP2) showed the strongest phosphate-solubilizing capacity (459.64 mg·L–1), with high nitrogen-fixation (53.12 mg·g–1) and IAA production (97.54 μg·mL–1). Inoculation with RP2 increased plant height and shoot fresh weight of tung tree by 68.5% and 98.2%, respectively, and root phosphorus content by 88.6%. Serratia marcescens (RP3) exhibited both phosphate-solubilization (399.10 mg·L–1). RP2 and Bacillus subtilis (SP4) had both phosphate solubilizing and antagonistic abilities against Fusarium oxysporum. Conclusion: Two multifunctional growth-promoting strains, RP2 and RP3, have been identified from tung tree for the first time. They significantly promote the growth of Vernicia species. This study provides core resources for developing green and environmentally friendly growth promoting agents.

Key words: Vernicia fordii, endophytic bacteria, phosphate solubilization, growth promotion, Burkholderia, Serratia

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