毛白杨翻译起始因子基因PtoeIF5A2的克隆及其表达特性分析

Abstract

Abstract:

Eukaryotic translation initiation factor 5A (eIF5A) plays an important role in plant development and various stresses. In this study, the full length PtoeIF5A2 (GenBank Accession number: HQ529480) cDNA was isolated from the cDNA library prepared from 2-month-old Populus tomentosa‘BJHR01’seedlings using RT-PCR technique. The PtoeIF5A2 coding region contained 483 nucleotides, encoding 160 amino acids with the molecular weight of about 18 kDa and PI of 5.76. The real time quantitative RT-PCR displayed that the expression of PtoeIF5A2 was highest in leaves in 6-month-old seedlings, about 5 times higher than that of in root. Moreover, the expression of PtoeIF5A2 in P. tomentosa responded to the drought, ABA and low temperature, especially NaCl treatment. After 24 h under NaCl, the PtoeIF5A2 expression was 25 times higher than control, and the PtoeIF5A2 promoter contained 6 response elements to pathogen and salinity, indicating that PtoeIF5A2 would be mainly involved in response of salt stress.

Key words: eIF5A, Populus tomentosa, salt stress, PtoeIF5A2

CLC Number:

S718.46

Guan Yang, Wang Hongzhi, Zhang Jiewei, Chen Yajuan, Zhu Dan, Ding Liping, Wei Jianhua. Isolation and Expression of Eukaryotic Translation Initiation Factor 5A2 in Populus tomentosa[J]. Scientia Silvae Sinicae, 2014, 50(2): 63-69.

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Isolation and Expression of Eukaryotic Translation Initiation Factor 5A2 in Populus tomentosa

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