银杏营养贮藏蛋白质的分离纯化与结构鉴定

Abstract

Abstract:

To reveal the relationship between the structure and function of vegetative storage proteins in Ginkgo biloba, the vegetative storage proteins were separated and purified by DEAE-Sepharose FF ion-exchange chromatography and Sephadex G-75 gel chromatography. A target protein was obtained and its molecule weight was 36 kDa. The mass-spectrometry showed that there were two peptide sequences which were consistent to some sequences of the Soybean storage protein (29 kDa) and the Dioscorea storage protein (30 kDa). The infrared spectrometry indicated that the secondary structure of the protein was mainly in β-sheet conformations.

Key words: Ginkgo biloba, vegetative storage protein, isolation and purification, structure identification

CLC Number:

S718.43

Wu Cai'e, Fan Gongjian, Li Tingting, Wu Haixia, Xu Wenbin. Isolation, Purification and Structural Determination of Vegetative Storage Proteins in Ginkgo biloba[J]. Scientia Silvae Sinicae, 2014, 50(2): 37-41.

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Isolation, Purification and Structural Determination of Vegetative Storage Proteins in Ginkgo biloba

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