花椒麻味素(酰胺类)含量的常规检测

Abstract

Abstract:

The unique numbing components in prickly ash (Zanthoxylum) consist of 8 different types of chained unsaturated fatty acid acylamides. It is meaningful to establish a rapid determination method of the numbing components contents (total acylamides) in prickly ash in evaluating the quality of prickly ash and its products. A three-levels and four-factors orthogonal experiment was conducted. The optimum ultrasonic-assisted extraction conditions for the total acylamides extract were in a 95% ethanol solvent with a 1:10 solid-liquid ratio at 50 ℃ for 2.5 h. Through a comparison study of the rapid formaldehyde titration method with HPLC approach for detecting the total acylamides contents in prickly ash, the adjusted coefficient of determination with the quick formaldehyde titration method was 2.269 6 (R²= 0.978 1). In addition, the rapid formaldehyde titration assay was used to detect the total acylamides contents of 14 types of prickly ash from different habitats. The results indicated that the Z.bungeanum, grown in alpine regions, had the highest total acylamides contents, followed by Z.armatum and then Z.bungeanum, found in low-relief terrain.

Key words: prickly ash, numbing components, acylamides, ultrasonic-assisted extraction, formaldehyde titration assay

CLC Number:

S713

Li Feifei, Li Menglou, Cui Jun, Gao Jinming. Conventional Determination of the Contents of Numbing Components (Acylamides) in Prickly Ash[J]. Scientia Silvae Sinicae, 2014, 50(2): 121-126.

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Conventional Determination of the Contents of Numbing Components (Acylamides) in Prickly Ash

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