Abstract:

Leaves and stem segments of P.×euramricana transformaed with A.Tumefaciens hauboring binary vectors pB48.214 and pB48.215 containing chimeric genes of 35S-Bt-Nos with different lengths of B.t gene respectively.Three plants selected by southern blot analysis showed IPC toxicity against larvae of Lymantria dispar.Based on the results of PCR analysis, southern blot of PCR products and genomic DNA as well as insect bioassay, three transformed plants were confirmed to be insect-resistant transgenic plants of P.×euramericana

Key words: P.×euramericana, B.t δ-endotoxin gene, Insect-resistang transgenic plants