银杉愈伤组织诱导植株再生的影响因素

doi:10.11707/j.1001-7488.LYKX20230141

Abstract

Abstract:

Objective: Cathaya argyrophylla is a rare and endangered plant unique to China, however it is difficult to breed. In this study, the factors influencing the induction callus of plant regeneration from mature embryo of C. argyrophylla were studied, so as to provide assistance in further elucidating the mechanisms that influence the induction of callus and adventitious buds in C. argyrophylla. Method: The mature embryos of C. argyrophylla from two provenances in Jinxiu and Guilin, Guangxi, were used as experimental materials. The media of 1/2 MS, DCR, and P6, with adding 0.5, 1.0, and 1.5 mg·L^?16-BA, 0.2, 0.4, and 0.6 mg·L^?1NAA, and 0.5, 1.0, 1.5 mg·L^?1IBA, respectively, were used to induce callus tissue, adventitious buds, and roots of C. argyrophylla, in order to obtain regenerated C. argyrophylla plants. The effects of two provenances, culture medium, plant growth regulators, etc. on the induction rate of callus, adventitious buds, adventitious roots, and proliferation rate of breeding and strengthening buds of C. argyrophylla were analyzed. Result: The callus induction rate, adventitious root induction rate, and breeding and strengthening buds rate of C. argyrophylla from Guilin provenance were significantly higher, with values of 66.48%, 21.43%, and 55.00%, respectively. The induction rate of adventitious buds in the C. argyrophylla from Jinxiu provenance was significantly higher, at 73.33%. The suitable culture medium and plant growth regulators for inducing plant regeneration from C. argyrophylla callus were: DCR+0.5 mg·L^?16-BA for callus induction, DCR+1.5 mg·L^?16-BA for adventitious bud induction, 1/2 MS+0.2 mg·L^?1NAA+0.5 mg·L^?1IBA for adventitious root induction, and DCR+1.0 mg·L^?16-BA+0.4 mg·L^?1NAA for bud proliferation and strengthening. Conclusion: The overall induction and proliferation rates of C. argyrophylla from Guilin, Guangxi are higher than those from Jinxiu, Guangxi. DCR medium has a significant promoting effect on the induction of callus and adventitious buds in C. argyrophylla, while 1/2 MS medium has a significant promoting effect on the induction of adventitious roots of C. argyrophylla. The 6-BA can induce both callus tissue and adventitious buds of C. argyrophylla. The combination of NAA and IBA can induce adventitious roots of C. argyrophylla. The combination of 6-BA and NAA can proliferate and strengthen adventitious buds of C. argyrophylla.

Key words: Cathaya argyrophylla, endangered species, mature embryo, callus, adventitious bud induction, plant regeneration

CLC Number:

Kaile Mai,Rongzhen Li,Hong Liu,Kun Qin,Jianhua Lan,Changsan Zhu,Lixin Feng,Jienan Su,Quanlian Zhou. Factors Influencing the Callus Induction and Plant Regeneration of Cathaya argyrophylla[J]. Scientia Silvae Sinicae, 2024, 60(7): 56-64.

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References 0

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指标 Index	植物生长调节剂 Growth regulator/(mg·L^?1)	金秀种源 Jinxiu provenance(%)			桂林种源 Guilin provenance(%)
指标 Index	6-BA	1/2 MS	DCR	P6	1/2 MS	DCR	P6
愈伤率 Callus induction rate	0.5	15.56±8.68abc	14.81±14.81abc	59.72±14.50bc	47.22±23.73abc	66.48±11.11c	24.61±4.45abc
	1.0	30.16±8.40abc	8.93±4.49ab	36.62±16.14abc	60.32±24.95bc	20.83±4.17abc	9.78±7.71ab
	1.5	23.61±11.87abc	0.00±0.00a	50.07±17.71abc	58.33±30.05bc	43.33±23.33abc	13.73±2.29ab
不定芽诱导率 The induction rate of adventitious bud	0.5	37.78±6.19abc	42.96±12.92abc	28.24±16.44abc	25.00±14.43ab	7.41±7.41a	39.89±10.63abc
	1.0	30.16±8.40abc	48.21±5.74abc	29.08±15.90abc	25.40±12.99ab	45.83±4.17abc	45.29±10.62abc
	1.5	29.167±18.16abc	73.33±6.67c	32.15±18.91abc	20.83±20.83a	26.67±26.67abc	69.22±1.38bc
干枯率 Shriveling rate	0.5	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a	3.53±1.85a
	1.0	0.00±0.00a	0.00±0.00a	5.80±2.90ab	0.00±0.00a	16.67±8.33b	2.90±2.90a
	1.5	11.11±11.11ab	0.00±0.00a	5.93±1.58ab	8.33±8.33ab	0.00±0.00a	4.62±0.14ab
未发育率Undeveloped rate	0.5	46.67±3.33c	42.22±8.89bc	12.04±6.48ab	27.78±14.70abc	26.11±3.89abc	30.93±12.69abc
	1.0	39.68±3.18bc	42.86±7.14bc	28.50±2.41abc	14.29±14.29ab	16.67±16.67abc	42.03±7.97bc
	1.5	36.11±1.39abc	26.67±6.67abc	11.86±3.16ab	12.50±12.50ab	30.00±10.00abc	7.66±4.16a

指标Index	植物生长调节剂 Growth regulator/(mg·L^?1)		金秀种源 Jinxiu provenance(%)			桂林种源 Guilin provenance(%)
指标Index	6-BA	NAA	1/2 MS	DCR	P6	1/2 MS	DCR	P6
愈伤率 Callus induction rate	0.5	0.2	100.00±0.00g	31.94±9.11bcd	71.77±9.05ef	100.00±0.00g	25.00±0.00abc	86.67±6.67fg
	1.0	0.4	60.92±8.57ef	57.77±11.28de	33.63±11.25bcd	0.00±0.00a	10.00±0.00ab	25.00±0.00abc
	1.5	0.6	66.67±16.67ef	12.26±6.57ab	48.96±12.20cde	0.00±0.00a	14.76±8.66ab	16.67±16.67ab
不定芽增殖率 Bud proliferation and strengthen rate	0.5	0.2	0.00±0.00a	50.28±0.28g	12.33±2.26abc	0.00±0.00a	35.00±0.00def	0.00±0.00a
	1.0	0.4	6.10±0.51ab	23.72±1.89cd	14.13±1.30bc	20.00±0.00c	55.00±0.00g	0.00±0.00a
	1.5	0.6	33.33±16.67de	46.23±2.07fg	24.85±0.77cd	0.00±0.00a	44.84±2.60efg	48.89±0.56g
干枯率 Shriveling rate	0.5	0.2	0.00±0.00a	17.78±8.94ab	15.90±8.25ab	0.00±0.00a	40.00±0.00bc	13.33±6.67ab
	1.0	0.4	24.03±1.51ab	12.90±7.23ab	8.86±4.44ab	80.00±0.00d	35.00±0.00abc	75.00±0.00d
	1.5	0.6	0.00±0.00a	21.15±3.44ab	21.03±14.30ab	66.67±33.33cd	23.37±11.30ab	34.45±17.22abc
未发育率 Undeveloped rate	0.5	0.2	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a
	1.0	0.4	0.00±0.00a	5.61±5.61ab	43.39±13.76c	0.00±0.00a	0.00±0.00a	0.00±0.00a
	1.5	0.6	0.00±0.00a	20.36±6.51abc	5.16±2.60ab	33.33±33.33bc	17.02±5.82abc	0.00±0.00a

植物生长调节剂Growth regulator/(mg·L^?1)		金秀种源Jinxiu provenance(%)			桂林种源Guilin provenance(%)
IBA	NAA	1/2 MS	DCR	P6	1/2 MS	DCR	P6
0.5	0.2	16.67±8.33b	0.00±0.00a	1.04±1.04a	21.43±3.57b	0.00±0.00a	1.04±1.04a
1.0	0.2	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a	0.00±0.00a
1.5	0.2	0.00±0.00a	0.00±0.00a	4.76±4.76a	0.00±0.00a	0.00±0.00a	4.76±4.76a

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Factors Influencing the Callus Induction and Plant Regeneration of Cathaya argyrophylla

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