Abstract:

Bacillus amyloliquefaciens JK-JS3, isolated from the leaf of the Pinus massoniana in Dr.Sun Yat-sen's mausoleum, is a bacterium and has high nematicidal activity to a nematode(Bursaphelenchus xylophilus). A water solution with the nematicidal activity was isolated and purified from the bacterium culture medium by acid and alkali sedimentation method, and the components was analyzed by gas chromatography and mass spectrum. The result showed that the nematicidal activity components was composed of three compounds which included Hexahydro-5-methyl-1-phenyl-1,3,5-triazine-2-thione, 2,2-dimethyl-N-phenylpropanethioamide and Semiarbazide,4-(1,7,7-trimethylbicyclo hepty1-2-ideno)-.The three compounds have not been reported to have the nematicidal activity before based on a search of information and patents of the three chemical compounds in the SciFinder.

Key words: Bursaphelenchus xylophilus, Bacillus amyloliquefaciens, nematicidal active substance, structure analysis