Abstract:

Proteins in healthy and infected leaves of diseased trees and healthy leaves of healthy trees of Paulownia tomentosa Steud and P.Fortunei(Seem)Hemsl,which were on the same direction and at the same age,related to Paulownia witche's broom were studied by SDS-PAGE and 2-D-PAGE.The results indicated that there existed difference among healthy and infected leaves of diseased trees and healthy tree leaves of P.tomentosa and P.Fortunei trees in protein quantity and quality,in which obvious protein numbers were 22,20,17 and 27,21,22 with SDS-PAGE respectively.Moreover,the changes of proteins in healthy and infected leaves of diseased trees and healthy tree leaves of P.tomentosa had similarity to that of P.Fortunei on basis of 2-D-PAGE.That is,the protein(pI 6.8,MW24KD)existed in healthy tree leaves and infected-healthy leaves of diseased trees disappeared in diseased leaves.This protein change might be considered to have relationship with Paulownia witche's broom.

Key words: Paulownia, Witche's broom, Protein, Electrophoresis