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Scientia Silvae Sinicae ›› 2018, Vol. 54 ›› Issue (8): 124-132.doi: 10.11707/j.1001-7488.20180814

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Determination of Individual Jujube Trees against Jujube Witches'-Broom Disease and Screening of Resistant Varieties from the Ancient Individual Jujube Trees Growing in Beijing

Wang He1, Ren Zhengguang2, Pan Yanping1, Feng Shukuai3, Lin Caili4, Chang Enzhong5, Yu Shaoshuai4, Tian Guozhong4   

  1. 1. Beijing Forestry Protection Station Beijing 100029;
    2. College of Plant Science and Technology, Beijing University of Agriculture Beijing 102206;
    3. Forestry and Plant Protection Station of Changping District of Beijing Beijing 102200;
    4. Key Laboratory of Forest Protection of State Forestry Administration Research Institute of Forest Ecology, Environment and Protection, CAF Beijing 100091;
    5. Forestry Protection and Quarantine Station of Huairou District of Beijing Beijing 101400
  • Received:2017-06-12 Revised:2018-02-12 Online:2018-08-25 Published:2018-08-18

Abstract: [Objective]This study aimed to understand the genetic resistance of jujube trees to jujube witches'-broom by investigating the resources of jujube trees in Beijing, which would provide basic data for the development and utilization of Jujube Witches' disease varieties.[Method]In this study, scions were collected from the healthy individual ancient jujube trees, and grafted onto the diseased jujube trees in a diseased jujube orchards to test the resistances to the jujube witches'-broom disease and screen the resistant varieties by the grafting inoculation method. [Result]As a result, 45 resistant scions with a certain resistance to the disease were screened out from 147 tested scions in Huairou district by tracking investigation since 2012, data statistical analysis, and PCR detection, and those resistant grafted scions displayed no or light symptoms, the spreading and propagation of phytoplasmas were slow, and the average disease index was low. A rescreening test was conducted in Changping district in 2013, a batch of strains with stable ability to resist jujube disease were determined from the above 45 scions according to the data of the monthly disease index survey. Combined with the survey results in 2014, 7 varieties derived from different scions were selected. Those varieties displayed normal growth and bearing fruits, and had good horticultural characteristics and potential utilization value. [Conclusion]This study has preliminarily clarified that the ancient jujube trees in Beijing have certain ability to resist Jujube Witches'-broom, and some disease-resistant cultivars have been screened, which lays a solid foundation for development and utilization of these resistant jujube varieties as well as for prevention and control of the disease.

Key words: ancient jujube, jujube witches'-broom disease, phytoplasma, resistant varieties, graft inoculation, pathogen detection

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