褐环乳牛肝菌与绿木霉复合接种对辽西北地区樟子松根系的影响

doi:10.11707/j.1001-7488.20180507

Abstract

Abstract: [Objective] Our study investigated the effects of single and combined inoculation with Suillus luteus and Trichoderma virens on the growth characteristics and root system architecture of Pinus sylvestris var. mongolica seedlings in the northwestern Liaoning Province, to reveal the regulation of rhizosphere microorganisms on seedling root system architecture.[Method] In this study, P. sylvestris var. mongolica seedlings grown in northwestern Liaoning were inoculated with the following four agents: 1. Sterile PD (potato, dextrose) culture medium (CK); 2. T. virens (T43); 3. S. luteus (N94); 4. S. luteus and T. virens (N94+T43). In one year after treatments, the mycorrhizal dependence, growth index, root topology architecture and fractal dimension of the seedlings under different treatment conditions were determined.[Result] The results showed that: 1) The mycorrhizal dependence under treatment of combined inoculation (N94+T43) was 84.91%, which was significantly higher than that of single inoculation and CK (P<0.05). 2)The improving effects of different treatments on height, collar diameter, fresh weight and dry weight of the seedlings were: N94+T43 > N94 > T43 > CK. 3) The root crown ratio under different treatments were: T43 > CK > N94 > N94+T43. 4) The combined inoculation (N94+T43) dramatically improved the root system parameters like root length, surface area, volume, average diameter, and tip and branch numbers of seedlings, which were significantly different from those of the other treatments (P<0.05). 5) The topology indexes under different treatments were approximate to 1, suggesting that the seedling root system architecture is a typical fishtail branch. The fractal dimensions of 2-year-old P. sylvestris var. mongolica seedlings roots were: N94+T43 > N94 > T43 > CK.[Conclusion] Under treatment of combined inoculation (N94+T43), the mycorrhizal dependence was the highest, while the root crown ratio was decreased. The combined inoculation promoted the seedling height, collar diameter, fresh weight and dry weight, and increased the root parameters such as root length and surface area were significantly compare to those of other treatments (P<0.05). All treatments did not change the fishtail branch pattern of the roots, but the fractal dimension under combinede inoculation treatment was the largest. The results demonstrate that combined inoculation is more effective in changing the root system architecture than other treatments.

Key words: Pinus sylvestris var, mongolica, Suillus luteus, Trichoderma virens, root architecture

CLC Number:

S763

Qi Jinyu, Song Ruiqing. Effects of Combined Inoculation with Suillus luteus and Trichoderma virens on the Roots of Pinus sylvestris var. mongolica in Northwest Liaoning Province[J]. Scientia Silvae Sinicae, 2018, 54(5): 62-69.

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Effects of Combined Inoculation with Suillus luteus and Trichoderma virens on the Roots of Pinus sylvestris var. mongolica in Northwest Liaoning Province

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